研究发现肠道微生物群与肺癌风险相关。然而，其与各种类型肺癌的因果关系仍不清楚。我们利用 MiBioGen 联盟迄今为止最大的肠道微生物群数据全基因组关联分析进行了孟德尔随机化 (MR) 研究，并汇总了各种类型肺癌的统计数据来自肺癌跨学科研究、国际肺癌联盟和 FinnGen 联盟 R7 发布的数据。采用逆方差加权、加权模型、MR-Egger 回归和加权中位数来评估肠道微生物群与各种类型肺癌之间的因果关系。敏感性分析用于测试工具变量中是否存在多效性和异质性。对这些细菌进行了反向磁共振分析，以确定它们在引起肺癌中的潜在作用。对这些细菌进行了反向磁共振分析，以确定它们在引起肺癌中的潜在作用。采用多变量孟德尔随机化 (MVMR) 来评估肠道微生物群对各种类型肺癌风险的直接因果影响。使用 IVW 作为主要分析方法，我们总共鉴定了 40 组肠道微生物群，这些微生物群与肺癌存在潜在因果关系。肺癌的各种亚型，其中10种与肺癌相关，10种与肺腺癌相关，9种与鳞状细胞肺癌相关，11种与小细胞肺癌相关的细菌。进行FDR校正后，我们进一步发现消化球菌科与肺腺癌之间仍然存在显着的因果关系。敏感性分析证明了这些结果的稳健性，没有发现异质性或多效性。我们的结果证实了特定肠道微生物群与肺癌之间的因果关系，为肠道微生物群在介导肺癌发展中的作用提供了新的见解。版权所有 © 2023李、王、张、杨、吴、赵。

The gut microbiota has been found to be associated with the risk of lung cancer. However, its causal relationship with various types of lung cancer remains unclear.We conducted a Mendelian randomization (MR) study using the largest genome-wide association analysis of gut microbiota data to date from the MiBioGen consortium, with pooled statistics for various types of lung cancer from the Transdisciplinary Research in Cancer of the Lung, the International Lung Cancer Consortium, and FinnGen Consortium R7 release data. Inverse variance weighted, weighted model, MR-Egger regression, and weighted median were adapted to assess the causal relationship between gut microbiota and various types of lung cancer. Sensitivity analysis was used to test for the presence of pleiotropy and heterogeneity in instrumental variables. A reverse MR analysis was performed on these bacteria to determine their potential role in causing lung cancer. A reverse MR analysis was performed on these bacteria to determine their potential role in causing lung cancer. Multivariable Mendelian randomization (MVMR) was conducted to assess the direct causal impact of gut microbiota on the risk of various types of lung cancer.Using IVW as the primary analytical method, we identified a total of 40 groups of gut microbiota with potential causal associations with various subtypes of lung cancer, of which 10 were associated with lung cancer, 10 with lung adenocarcinoma, 9 with squamous cell lung cancer, and 11 groups of bacteria associated with small cell lung cancer. After performing FDR correction, we further found that there was still a significant causal relationship between Peptococcaceae and lung adenocarcinoma. Sensitivity analyses demonstrated the robustness of these results, with no heterogeneity or pleiotropy found.Our results confirm a causal relationship between specific gut microbiota and lung cancer, providing new insights into the role of gut microbiota in mediating the development of lung cancer.Copyright © 2023 Li, Wang, Zhang, Yang, Wu and Zhao.