从巴戟天叶子中分离并充分表征了四种未描述的双环烯醚萜苷（称为 phuketosides A-D）和一种环烯醚萜苷（称为 phuketoside E）。植物化学分析还揭示了八种已知化合物的存在。通过对 1D 和 2D-​​NMR 光谱以及 HRMS 光谱数据的广泛分析确定了结构，并通过 ECD 计算推导出分离物的绝对构型。提出了通过分子间 Diels-Alder 型反应产生双环烯醚萜苷、普酮苷 A-C 的生物遗传途径。除 phuketosides B 和 D 外，分离的化合物针对一组癌细胞系（MOLT-3、HuCCA-1、A549、HeLa、HepG2 和 MDA-MB-231）和非癌细胞进行了评估线（MRC-5）的细胞毒性。所有分离株均未对测试的细胞系产生显着的细胞毒性作用。版权所有 © 2023。由 Elsevier Ltd 出版。

Four undescribed bis-iridoid glycosides, named phukettosides A-D, and one iridoid glycoside, referred to as phukettoside E, were isolated and fully characterized from the leaves of Morinda umbellata L. Phytochemical analysis also revealed the presence of eight known compounds. The structures were determined through extensive analysis of 1D and 2D-NMR spectroscopic and HRMS spectral data, and the absolute configurations of the isolates were deduced through ECD calculations. Biogenetic pathways for the bis-iridoid glycosides, phukettosides A-C, through intermolecular Diels-Alder type reactions, were proposed. The isolated compounds, with the exception of phukettosides B and D, were evaluated against a panel of cancer cell lines (MOLT-3, HuCCA-1, A549, HeLa, HepG2, and MDA-MB-231) and a non-cancerous cell line (MRC-5) for their cytotoxicity. None of the isolates had significant cytotoxic effects on the tested cell lines.Copyright © 2023. Published by Elsevier Ltd.