本文报告了一项研究的结果，该研究旨在使用气相色谱-质谱 (GC-MS) 分析来鉴定和表征罗勒提取物的成分，并评估罗勒配制的纳米乳液的理化性质和稳定性提炼。 GC-MS 分析显示，O. basilicum 提取物含有 22 种成分，其中石竹烯和柚皮素被确定为主要活性成分。该纳米乳液制剂具有小且均匀的粒径分布、负zeta电位以及对O. basilicum提取物的高包封率，在生物医学领域具有良好的应用潜力。纳米乳液呈现球形形态，并在长达 6 个月内保持物理稳定。体外释放研究表明，与游离提取物溶液相比，纳米乳液制剂中的提取物具有持续释放。此外，所开发的纳米制剂对 K562 细胞表现出增强的抗癌特性，同时在正常细胞 (HEK293) 中表现出低毒性。 O. basilicum 提取物对铜绿假单胞菌、白色念珠菌和表皮葡萄球菌具有抗菌活性，与纳米乳液结合时观察到潜在的协同效应。这些发现有助于了解罗勒提取物及其纳米乳液配方在医疗保健和制药行业等各个领域的成分和潜在应用。需要进一步优化和研究，以最大限度地提高提取物及其纳米制剂的功效和抗菌活性。研究亮点：本研究表征了O. basilicum 提取物的成分，并评估了其纳米乳液配方的理化性质和稳定性。 O. basilicum 提取物含有 22 种成分，其中石竹烯和柚皮素被确定为主要活性成分。该纳米乳液制剂在生物医学应用方面表现出巨大的潜力，具有提取物的持续释放、低毒性以及增强的抗癌和抗菌特性。这些发现有助于了解 O. basilicum 提取物及其纳米乳液制剂在医疗保健和制药行业中的潜在应用，强调了进一步优化和研究的必要性。© 2023 Wiley periodicals LLC。

This paper reports on the findings from a study that aimed to identify and characterize the constituents of Ocimum basilicum extract using gas chromatography-mass spectrometry (GC-MS) analysis, as well as assess the physicochemical properties and stability of nanoemulsions formulated with O. basilicum extract. The GC-MS analysis revealed that the O. basilicum extract contained 22 components, with Caryophyllene and Naringenin identified as the primary active constituents. The nanoemulsion formulation demonstrated excellent potential for use in the biomedical field, with a small and uniform particle size distribution, a negative zeta potential, and high encapsulation efficiency for the O. basilicum extract. The nanoemulsions exhibited spherical morphology and remained physically stable for up to 6 months. In vitro release studies indicated sustained release of the extract from the nanoemulsion formulation compared to the free extract solution. Furthermore, the developed nanoformulation exhibited enhanced anticancer properties against K562 cells while demonstrating low toxicity in normal cells (HEK293). The O. basilicum extract demonstrated antimicrobial activity against Pseudomonas aeruginosa, Candida albicans, and Staphylococcus epidermidis, with a potential synergistic effect observed when combined with the nanoemulsion. These findings contribute to the understanding of the constituents and potential applications of O. basilicum extract and its nanoemulsion formulation in various fields, including healthcare and pharmaceutical industries. Further optimization and research are necessary to maximize the efficacy and antimicrobial activity of the extract and its nanoformulation. RESEARCH HIGHLIGHTS: This study characterized the constituents of O. basilicum extract and assessed the physicochemical properties and stability of its nanoemulsion formulation. The O. basilicum extract contained 22 components, with Caryophyllene and Naringenin identified as the primary active constituents. The nanoemulsion formulation demonstrated excellent potential for biomedical applications, with sustained release of the extract, low toxicity, and enhanced anticancer and antimicrobial properties. The findings contribute to the understanding of the potential applications of O. basilicum extract and its nanoemulsion formulation in healthcare and pharmaceutical industries, highlighting the need for further optimization and research.© 2023 Wiley Periodicals LLC.