基于微流体的纳米乳液的纳米乳液：成分，稳定性，表征和潜在的生物医学应用，以改善抗菌和抗癌特性

本文报告了一项旨在使用气相色谱 - 质谱法（GC-MS）分析的研究结果的发现，该研究旨在识别和表征Ocimum Basilicum提取物的组成部分，并评估用O. basilicum提取物制成的纳米乳液的物理化学特性和稳定性。 GC-MS分析表明，O. basilicum提取物含有22个成分，其股骨和纳林宁被鉴定为主要活性成分。纳米乳液配方在生物医学领域具有极大的使用潜力，其粒径分布较小且均匀，负ZETA电势和高质质提取物的高包封效率。纳米乳液表现出球形形态，并且在物理上保持稳定长达6个月。体外释放研究表明，与游离提取溶液相比，纳米乳液制剂中提取物的持续释放。此外，开发的纳米成型表现出对K562细胞的抗癌特性增强，同时表现出正常细胞中毒性较低（HEK293）。 O. basilicum提取物表现出针对铜绿假单胞菌，白色念珠菌和葡萄球菌表皮的抗菌活性，当与纳米乳液结合时观察到潜在的协同作用。这些发现有助于理解O. basilicum提取物及其在包括医疗保健和制药行业在内的各个领域的纳米乳液的成分和潜在应用。进一步的优化和研究对于提取提取物及其纳米成型的功效和抗菌活性是必要的。研究重点：这项研究表征了O. basilicum提取物的组成部分，并评估了其纳米乳液配方的理化特性和稳定性。 O. basilicum提取物含有22个成分，其中具有股骨和纳林宁为主要活性成分。纳米乳剂配方在生物医学应用中表现出巨大的潜力，并持续释放提取物，低毒性以及增强的抗癌和抗菌特性。这些发现有助于理解O. basilicum提取物及其在医疗保健和制药行业中的纳米乳液的潜在应用，从而强调了进一步优化和研究的需求。

This paper reports on the findings from a study that aimed to identify and characterize the constituents of Ocimum basilicum extract using gas chromatography-mass spectrometry (GC-MS) analysis, as well as assess the physicochemical properties and stability of nanoemulsions formulated with O. basilicum extract. The GC-MS analysis revealed that the O. basilicum extract contained 22 components, with Caryophyllene and Naringenin identified as the primary active constituents. The nanoemulsion formulation demonstrated excellent potential for use in the biomedical field, with a small and uniform particle size distribution, a negative zeta potential, and high encapsulation efficiency for the O. basilicum extract. The nanoemulsions exhibited spherical morphology and remained physically stable for up to 6 months. In vitro release studies indicated sustained release of the extract from the nanoemulsion formulation compared to the free extract solution. Furthermore, the developed nanoformulation exhibited enhanced anticancer properties against K562 cells while demonstrating low toxicity in normal cells (HEK293). The O. basilicum extract demonstrated antimicrobial activity against Pseudomonas aeruginosa, Candida albicans, and Staphylococcus epidermidis, with a potential synergistic effect observed when combined with the nanoemulsion. These findings contribute to the understanding of the constituents and potential applications of O. basilicum extract and its nanoemulsion formulation in various fields, including healthcare and pharmaceutical industries. Further optimization and research are necessary to maximize the efficacy and antimicrobial activity of the extract and its nanoformulation. RESEARCH HIGHLIGHTS: This study characterized the constituents of O. basilicum extract and assessed the physicochemical properties and stability of its nanoemulsion formulation. The O. basilicum extract contained 22 components, with Caryophyllene and Naringenin identified as the primary active constituents. The nanoemulsion formulation demonstrated excellent potential for biomedical applications, with sustained release of the extract, low toxicity, and enhanced anticancer and antimicrobial properties. The findings contribute to the understanding of the potential applications of O. basilicum extract and its nanoemulsion formulation in healthcare and pharmaceutical industries, highlighting the need for further optimization and research.