结肠腺癌（COAD）是全球最常见的癌症之一，在发病率和死亡率方面位居第三位。生长抑素受体 (SSTR) 家族包括 G 蛋白偶联受体 (GPCR)，其在与生长抑素 (SST) 类似物结合后与抑制性 G 蛋白（Gi 和 Go）偶联。 GPCR 参与激素释放、神经传递、细胞生长抑制和癌症抑制。然而，他们在 COAD 中的角色仍不清楚。本研究利用生物信息学研究了 COAD 中 SSTR 家族成员的表达、预后、基因改变、功能富集和免疫调节作用。 SSTR1-4 在 COAD 中差异表达下调，低 SSTR2 表达表明生存率较差。使用京都基因和基因组百科全书以及基因本体论进一步分析了 COAD 中 SSTR 家族的生物学过程和基因表达富集。观察到 SSTR 表达与免疫细胞浸润之间存在很强的相关性。我们还使用定量实时聚合酶链反应定量了 25 个 COAD 样本和邻近正常组织中的 SSTR2 表达。我们分析了它与树突状细胞整合素亚基αX标记基因的相关性。实体瘤门户的生物标志物探索用于确认 SSTR2 与免疫调节剂和免疫治疗反应之间的相关性。我们的结果表明 SSTR2 是 COAD 免疫治疗的一个有前途的靶点。我们的研究结果为 SSTR 家族的生物学功能及其对 COAD 预后的影响提供了新的见解。版权所有 © 2023 Yu、Yang、Nie、Jiang、He 和 Ou。

Colon adenocarcinoma (COAD) is among the most prevalent cancers worldwide, ranking as the third most prevalent malignancy in incidence and mortality. The somatostatin receptor (SSTR) family comprises G-protein-coupled receptors (GPCRs), which couple to inhibitory G proteins (Gi and Go) upon binding to somatostatin (SST) analogs. GPCRs are involved in hormone release, neurotransmission, cell growth inhibition, and cancer suppression. However, their roles in COAD remain unclear. This study used bioinformatics to investigate the expression, prognosis, gene alterations, functional enrichment, and immunoregulatory effects of the SSTR family members in COAD. SSTR1-4 are differentially downregulated in COAD, and low SSTR2 expression indicates poor survival. Biological processes and gene expression enrichment of the SSTR family in COAD were further analyzed using the Kyoto Encyclopedia of Genes and Genomes and Gene Ontology. A strong correlation was observed between SSTR expression and immune cell infiltration. We also quantified SSTR2 expression in 25 COAD samples and adjacent normal tissues using quantitative real-time polymerase chain reaction. We analyzed its correlation with the dendritic cell-integrin subunit alpha X marker gene. The biomarker exploration of the solid tumors portal was used to confirm the correlation between SSTR2 with immunomodulators and immunotherapy responses. Our results identify SSTR2 as a promising target for COAD immunotherapy. Our findings provide new insights into the biological functions of the SSTR family and their implications for the prognosis of COAD.Copyright © 2023 Yu, Yang, Nie, Jiang, He and Ou.