生物活性多糖是高效预防骨病的一种有前景的方法。裂褶多糖（SPG）是一种从真菌中提取的多糖，具有抗癌、抗肿瘤和抗炎作用。在本研究中，首次通过体外测定评估了在 SPG 上生长的人脂肪组织源性间充质干细胞 (hADMSC) 的细胞增殖、成骨标志物、矿物质沉积和成骨基因表达。使用MTT测定和吖啶橙染色进行测量。使用碱性磷酸酶 (ALP) 活性测试、细胞钙含量测定和矿化基质染色来评估 hADMSC 的分化。为此，通过实时 RT-PCR 在两周内使用茜素红 S、von Kossa 染色以及骨特异性标记物（包括 ALP、Runx2 和骨连接蛋白）的表达。根据结果​​，确定10μg/ml浓度的SPG作为分化研究的最佳剂量。体外成骨分化试验结果表明，与体外对照组相比，SPG增强了分化hADMSCs在分化过程中的成骨标志物和矿化作用，并上调了骨特异性基因的表达。结果表明，SPG可以作为有效的应用未来成骨分化的因素。当前的研究提供了对基于 hADMSC 的治疗的见解，并为患有骨缺陷和损伤的个体引入了有前景的治疗材料。© 2023。作者获得 Springer Nature B.V. 的独家许可。

Bioactive polysaccharides are a promising way for bone disease prevention with high efficiency. Schizophyllan (SPG) is a polysaccharide derived from a species of fungus with anticancer, antitumor, and anti-inflammatory effects. In the present study, for the first time, the cell proliferation, osteogenic markers, mineral deposition, and osteogenic gene expression of human adipose tissue-derived mesenchymal stem cells (hADMSCs) grown on SPG were evaluated by in vitro assays.The cytotoxicity of SPG was measured using the MTT assay and acridine orange staining. Differentiation of hADMSCs was assessed using alkaline phosphatase (ALP) activity test, cellular calcium content assay, and mineralized matrix staining. To this end, Alizarin red S, von Kossa staining, and the expression of bone-specific markers, including ALP, Runx2, and osteonectin, were used by real-time RT-PCR over a 2-week period. According to the results, SPG at 10 µg/ml concentration was determined as the optimal dosage for differentiation studies. The results of osteogenic differentiation tests showed that compared to the control groups in vitro, SPG enhanced the osteogenic markers and mineralization as well as upregulation of the expression of bone specific genes in differentiated hADMSCs during differentiation.The results revealed that SPG could be applied as effective factor for osteogenic differentiation in the future. The current study provides insights into the hADMSC-based treatment and introduces promising therapeutic material for individuals who suffer from bone defects and injuries.© 2023. The Author(s), under exclusive licence to Springer Nature B.V.