膀胱癌是影响泌尿道的最常见恶性肿瘤。遗传改变与膀胱癌的发展密切相关。 MicroRNA (miRNA) 是一种小型非编码单链 RNA 分子，与膀胱癌有关。 miR-124-3pa 在各种类型的人类恶性肿瘤中表现出表达改变。 DNA 甲基转移酶 3B (DNMT3B) 负责 DNA 从头甲基化，这是致癌作用中的基本表观遗传过程。本工作旨在研究膀胱癌组织中DNMT3B和miR 124-3pa的表达情况，探讨其在疾病诊断和预后中的意义。本病例对照研究纳入了106例原发性尿路上皮患者的组织样本。膀胱癌。将组织分成两部分。第一部分立即冷冻并保存在 - 80°C 下，用于提取总 RNA，随后检测 miR 124-3pa 和 DNMT3B 表达。另一部分保存在福尔马林溶液中进行组织病理学检查。癌组织和正常组织中miRNA-124-3pa和DNMT3B的表达量均具有高度统计学差异(P<0.001)。此外，miRNA-124-3pa 和 DNMT3B 表达之间存在高度统计学显着的强负相关（r=-0.750，P < 0.001）。 miR-124-3pa和DNMT3B的综合表现表明，≥3.3的截止点可以作为膀胱癌存在的预测因子，敏感性为98.1%，特异性为80%。miR-124-3pa和DNMT3B可以可用作膀胱癌存在的预测因子。© 2023。作者获得 Springer Nature B.V. 的独家许可。

Cancer bladder is the most common malignant tumor affecting the urinary tract. Genetic alterations are tightly associated with the development of cancer bladder. MicroRNAs (miRNA) are small, noncoding single-stranded RNA molecules that have been linked to bladder cancer. miR-124-3pa exhibits altered expression in various types of human malignancies. DNA methyltransferase 3B (DNMT3B) is responsible for de novo DNA methylation which is a fundamental epigenetic process in carcinogenesis. This work was performed to study the expression of DNMT3B and miR 124-3pa in bladder cancer tissues, and investigate their significance in the diagnosis and prognosis of the disease.This case-control study included one hundred and six tissue samples of patients with primary urothelial bladder cancer. The tissues were separated into two parts. The first part was immediately frozen and kept at - 80 °C for total RNA extraction with subsequent detection of miR 124-3pa and DNMT3B expressions. The other part was preserved in formalin solution for histopathological examination.There was a highly statistically significant difference between the cancerous and the normal tissues as regarding miRNA-124-3pa and DNMT3B expression (P < 0.001) for each. Also, there was a highly statistically significant strong negative correlation between miRNA-124-3pa and DNMT3B expression (r=-0.750, P < 0.001). The combined performance of miR-124-3pa and DNMT3B revealed that the cutoff point of ≥ 3.3 can be used as a predictor of the presence of cancer bladder with sensitivity of 98.1% and specificity of 80%.miR-124-3pa and DNMT3B can be used as predictors of the presence of cancer bladder.© 2023. The Author(s), under exclusive licence to Springer Nature B.V.