肿瘤和培养细胞中的染色体数量经常发生动态变化，这使治疗以及理解基因型-机械型关系变得复杂。在这里，我们使用活细胞“ChReporter”方法来识别具有单条染色体丢失的细胞，以更好地了解细胞形状、运动和生长的差异。我们关注标准肺癌细胞系，并首先显示保留 ChReporter 的克隆群体在细胞和核形态以及运动性方面表现出巨大差异。表型指标遵循简单的规则，包括随速度变化的迁移持久性，并且从药物反应、成像和单细胞 RNA 测序中可以明显看出细胞骨架的差异。然而，机械型-基因型关系被证明是复杂的，激发了对丢失或保留 5 号染色体 ChReporter 的克隆进行比较。当丢失时，无荧光细胞显示 5 号染色体基因的低表达，包括调节 MT 和增殖的关键肿瘤抑制因子 APC。集落紧凑，细胞核圆形，细胞增殖更多，药物结果暗示 APC，患者生存数据表明肺和其他肿瘤类型之间的关联。因此，使用 ChReporters 对基因型进行视觉识别可以帮助阐明机械类型和机械进化。

Chromosome numbers often change dynamically in tumors and in cultured cells, which complicates therapy as well as understanding genotype-mechanotype relationships. Here we use a live-cell 'ChReporter' method to identify cells with a single chromosomal loss in efforts to better understand differences in cell shape, motility, and growth. We focus on a standard lung cancer line and first show clonal populations that retain the ChReporter exhibit large differences in cell and nuclear morphology as well as motility. Phenotype metrics follow simple rules, including migratory persistence scaling with speed, and cytoskeletal differences are evident from drug responses, imaging, and single-cell RNA sequencing. However, a mechanotype-genotype relationship proves complex, motivating comparisons of clones that had either lost or retained a Chromosome-5 ChReporter. When lost, fluorescence-null cells show low expression of Chromosome-5 genes, including a key tumor suppressor APC that regulates MT's and proliferation. Colonies are compact, nuclei are rounded, and cells proliferate more, with drug results implicating APC, and patient survival data indicating an association in lung and other tumor-types. Visual identification of genotype with ChReporters can thus help clarify mechanotype and mechano-evolution.