溃疡性结肠炎（UC）代表一种临床上具有挑战性的疾病，其特征在于结肠上皮粘膜的持续损伤作为主要病理特征。聚乙烯醇（PVA）溶液主要由胶水组成，是一种可生物降解的聚合物材料，已在医疗领域得到应用。本研究致力于探讨 PVA 水溶液在改善小鼠 UC 方面的治疗潜力。通过在饮食中添加 2.5% DSS 6 天，在 48 只 C57BL/6 小鼠中诱导 UC。用不同浓度的 PVA（0.1 mg/ml PVA、0.3 mg/ml PVA、1 mg/ml PVA、3 mg/ml PVA、10 mg/ml PVA）灌肠处理小鼠（n = 6）。评估疾病活动指数（DAI）和组织学评分的炎症程度。此外，还培养了小鼠结肠类器官，用于评估 PVA 对体外扩张的影响。PVA 水溶液（1 mg/ml 和 3 mg/ml）能够减轻小鼠的 DAI。到第 6 天，1 mg/ml PVA 组的 DAI 显着降低了 3/5 倍 (p = 0.02)。组织病理学评分有所改善，同时肠粘膜组织中炎症因子的水平降低。此外，已证实 PVA 可以在体外促进结肠类器官的扩张。 总之，我们的研究结果表明，PVA 有可能改善患有 DSS 诱导结肠炎的小鼠受试者中与结肠炎相关的症状，主要是通过其促进肠道干细胞扩增。这项研究可能为溃疡性结肠炎的临床治疗提供新的候选者。© 2023。作者。

Ulcerative colitis (UC) represents a clinically challenging condition characterized by persistent damage to the colonic epithelial mucosa as the principal pathological feature. Polyvinyl alcohol (PVA) solution, primarily composed of glue, is a biodegradable polymer material that has found utility in the medical field. This research endeavors to investigate the therapeutic potential of PVA water solution in ameliorating UC in mice.UC was induced in 48 C57BL/6 mice by administering 2.5% DSS in their diet for 6 days. Mice were treated with different concentrations of PVA (0.1 mg/ml PVA, 0.3 mg/ml PVA, 1 mg/ml PVA, 3 mg/ml PVA, 10 mg/ml PVA) enemas (n = 6). Disease Activity Index (DAI) and histologic score were evaluated for inflammation degree. Furthermore, mouse colon organoids were cultured, which were used to assess the effects of PVA on expansion in vitro.PVA aqueous solutions (1 mg/ml and 3 mg/ml) were able to alleviate the DAI in mice. By DAY 6, there was a significant 3/5-fold decrease in DAI within the 1 mg/ml PVA group (p = 0.02). Histopathology scores demonstrated improvements, while the levels of inflammatory factors in the intestinal mucosal tissue were reduced. Additionally, it was confirmed that PVA could promote the expansion of colonic organoids in vitro.In summary, our investigation has yielded findings indicating that PVA holds the potential to ameliorate symptoms associated with colitis in murine subjects afflicted by DSS-induced colitis, primarily through its facilitation of intestinal stem cell expansion. This study might provide a new candidate for the clinical treatment of ulcerative colitis.© 2023. The Author(s).