芦丁减轻脂多糖诱导的山羊瘤胃上皮细胞损伤。
Rutin alleviated lipopolysaccharide-induced damage in goat rumen epithelial cells.
发表日期：2023 Oct 27
作者：
Jinshun Zhan, Zhiyong Gu, Haibo Wang, Yuhang Liu, Yanping Wu, Junhong Huo
来源：
ANTIOXIDANTS & REDOX SIGNALING
摘要：
芦丁，也称为维生素 P，是一种来自植物的黄酮类化合物。此前的研究表明，芦丁可以通过抑制氧化应激、改善炎症来减轻组织和细胞的损伤。目前尚未见芦丁对山羊瘤胃上皮细胞（GRECs）保护作用的报道。因此，我们研究了芦丁是否可以减轻脂多糖（LPS）诱导的GRECs损伤。GRECs在基础培养基或含有1μg/mL LPS、或1μg/mL LPS和20μg/mL芦丁的基础培养基中培养。每组进行六次重复。培养3 h后，收获GRECs检测相关参数。芦丁显着增强细胞活性（p < 0.05）和跨上皮电阻（TEER）（p < 0.01），显着降低细胞凋亡率（p < 0.05） LPS诱导的GREC。芦丁显着增加超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH-PX) 和过氧化氢酶 (CAT) 活性 (p<0.01)，并显着降低乳酸脱氢酶 (LDH) 活性以及活性氧 (ROS) 和丙二醛 (MDA) 水平LPS 诱导的 GREC（p<0.01）。在 LPS 诱导的 GREC 中，白细胞介素 6 (IL-6)、IL-1β 和 CXCL8 的 mRNA 和蛋白水平以及肿瘤坏死因子-α (TNF-α) 和 CCL5 的 mRNA 水平显着增加（p<0.05 或 p <0.01），而补充芦丁可显着降低 LPS 诱导的 GREC 中 IL-6、TNF-α 和 CXCL8 的 mRNA 和蛋白水平（p<0.05 或 p<0.01）。在 LPS 诱导的 GREC 中，Toll 样受体 2 (TLR2) 的 mRNA 水平以及 TLR4 和核因子 κB (NF-κB) 的 mRNA 和蛋白水平显着提高（p<0.05 或 p<0.01），而芦丁补充可以显着降低 TLR4 的 mRNA 和蛋白水平（p<0.05 或 p<0.01）。此外，芦丁有降低CXCL6、NF-κB和IκBα蛋白水平的趋势(0.05
Rutin, also called vitamin P, is a flavonoids from plants. Previous studies have indicated that rutin can alleviate the injury of tissues and cells by inhibiting oxidative stress and ameliorating inflammation. There has been no report on the protective effects of rutin on goat rumen epithelial cells (GRECs) at present. Hence, we investigated whether rutin can alleviate lipopolysaccharide (LPS)-induced damage in GRECs.GRECs were cultured in basal medium or basal medium containing 1 μg/mL LPS, or 1 μg/mL LPS and 20 μg/mL rutin. Six replicates were performed for each group. After 3-h culture, the GRECs were harvested to detect the relevant parameters.Rutin significantly enhanced the cell activity (p < 0.05) and transepithelial electrical resistance (TEER) (p<0.01) and significantly reduced the apoptosis rate (p<0.05) of LPS-induced GRECs. Rutin significantly increased superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and catalase (CAT) activity (p<0.01) and significantly decreased lactate dehydrogenase (LDH) activity and reactive oxygen species (ROS) and malondialdehyde (MDA) levels in LPS-induced GRECs (p<0.01). The mRNA and protein levels of interleukin 6 (IL-6), IL-1β and CXCL8 and the mRNA level of tumor necrosis factor-α (TNF-α) and CCL5 were significantly increased in LPS-induced GRECs (p<0.05 or p<0.01), while rutin supplementation significantly decreased the mRNA and protein levels of IL-6, TNF-α and CXCL8 in LPS-induced GRECs (p<0.05 or p<0.01). The mRNA level of toll-like receptor 2 (TLR2), and the mRNA and protein levels of TLR4 and nuclear factor κB (NF-κB) was significantly improved in LPS-induced GRECs (p<0.05 or p<0.01), whereas rutin supplementation could significantly reduce the mRNA and protein levels of TLR4 (p<0.05 or p<0.01). In additon, rutin had a tendency of decreasing the protein levels of CXCL6, NF-κB and IκBα (0.05