目的：研究 SIX4 在乳腺癌中的作用。方法：利用公开的在线工具分析SIX4在乳腺癌中的表达、甲基化和预后意义及其免疫组化。结果：高 SIX4 水平与低 SIX4 启动子甲基化相关，尤其是在雌激素受体阳性乳腺癌中。 SIX4 增加与晚期和免疫浸润减少有关。基因集富集分析发现，SIX4相关基因在转录加工和免疫反应方面富集。 SIX4 高表达的患者往往生存率较差，尤其是雌激素受体阳性乳腺癌患者。结论：乳腺癌中 SIX4 的高表达具有致癌作用，通过抑制免疫反应促进恶性肿瘤的发展，尤其是在管腔亚型中，并且与低启动子甲基化水平相关。

Aim: To investigate SIX4 in breast cancer. Methods: Publicly available online tools were used to analyze the expression, methylation and prognostic significance of SIX4 in breast cancer, as well as its immunohistochemistry. Results: High SIX4 levels were associated with low SIX4 promoter methylation, especially in estrogen receptor-positive breast cancer. Increased SIX4 was related to advanced stage and decreased immune infiltration. Gene set enrichment analysis found that the SIX4-correlated genes were enriched in transcriptional processing and immune response. Patients with high SIX4 expression tended to have poor survival, especially those with estrogen receptor-positive breast cancer. Conclusion: High SIX4 expression in breast cancer plays an oncogenic role, promoting the development of malignancies through suppressing the immune response, especially in luminal subtypes, and is associated with a low promoter methylation level.