上皮-间质转化（EMT）是上皮细胞和间质细胞短暂交换表型的能力。它在癌细胞中的识别与侵袭性临床表型相关。在肉瘤中，这种能力正在研究中。通过免疫组织化学评估儿科骨肉瘤中参与 EMT 的两种转录因子的表达及其与临床结果的关联。一项针对 18 岁以下骨肉瘤诊断儿童的回顾性队列研究。对诊断时收集的样本进行免疫组织化学检测 Snail 和 Twist-1 的表达。研究了免疫组织化学与临床结果和总生存率之间的相关性。纳入了 53 名患者。 Snail 表达有 26 例细胞质阳性病例 (49.1%)，并且与多发性转移瘤 (p = 0.02) 和远处骨转移 (p = 0.01) 的存在相关。另一方面，45例（84.9%）Twist-1在核位置表达呈阳性，与分析的临床变量无关。Snail和Twist-1在儿科骨肉瘤病例中频繁表达。细胞质 Snail 与多发性转移性疾病和远处骨转移的存在相关。两种标记物的阳性表明这些蛋白质作为该肿瘤中 EMT 事件的调节因子被激活，表明在与疾病临床表现相关的现象中发挥作用。

The epithelial-mesenchymal transition (EMT) is the ability of epithelial and mesenchymal cells to exchange phenotypes transiently. Its identification in carcinomatous cells has been associated with aggressive clinical phenotypes. In sarcomas, this ability is under study.to evaluate the expression of two transcription factors involved in EMT by immunohistochemistry in pediatric osteosarcoma and its association with clinical outcomes.A retrospective cohort study in children under 18 years of age with osteosarcoma diagnosis. Immunohistochemistry was performed for Snail and Twist-1 expressions from samples collected at the time of diagnosis. Correlations between immunohistochemistry and the clinical outcomes and overall survival were performed.53 patients were included. There were 26 positive cytoplasmic cases (49.1%) in Snail expression and were correlated with the presence of multiple metastases (p = 0.02) and distant bone metastases (p = 0.01). On the other hand, 45 cases (84.9%) were positive in Twist-1 expression in the nuclear location, showing no association with the analyzed clinical variables.Snail and Twist-1 were frequently expressed in pediatric cases of osteosarcoma. Cytoplasmic Snail was correlated with the presence of multiple metastatic disease and distant bone metastases. The positivity of both markers suggests the activation of these proteins as regulators of EMT events in this tumor, suggesting a role in the phenomena related to the clinical presentation of the disease.