癌症与细菌/真菌之间的关联已被广泛研究，但癌症相关病毒的影响尚未得到彻底研究。在这项研究中，我们全面表征了 31 种癌症类型的组织样本以及 23 种癌症类型的血液样本的癌症病毒组。我们的研究结果表明，在主要人类癌症的组织和血液中都存在低丰度的病毒 DNA，并且在不同癌症类型之间观察到病毒群落组成存在显着差异。此外，对头颈鳞状细胞癌 (HNSC)、肾透明细胞癌 (KIRC)、胃癌 (STAD) 和子宫体子宫内膜癌 (UCEC) 四种癌症进行的 Cox 回归分析显示，病毒感染与癌症之间存在很强的相关性。组织的组成/丰度和患者的存活率。此外，我们还确定了这四种癌症的病毒相关预后特征（VAPS），并发现了具有不同生存风险的患者中 VAPS 与组织中优势细菌之间相互作用的差异。值得注意的是，临床相关分析揭示了 VAPS 对这四种癌症的预后能力。总而言之，我们的研究为组织中病毒在多种癌症预后中的作用提供了新的见解，并为使用组织病毒对癌症患者的预后进行分层提供了指导。版权所有 © 2023 Elsevier Ltd. 保留所有权利。

The associations between cancer and bacteria/fungi have been extensively studied, but the implications of cancer-associated viruses have not been thoroughly examined. In this study, we comprehensively characterized the cancer virome of tissue samples across 31 cancer types, as well as blood samples from 23 cancer types. Our findings demonstrated the presence of viral DNA at low abundances in both tissue and blood across major human cancers, with significant differences in viral community composition observed among various cancer types. Furthermore, Cox regression analyses conducted on four cancers, including Head and Neck squamous cell carcinoma (HNSC), Kidney renal clear cell carcinoma (KIRC), Stomach adenocarcinoma (STAD), and Uterine Corpus Endometrial Carcinoma (UCEC), revealed strong correlation between viral composition/abundance in tissues and patient survival. Additionally, we identified virus-associated prognostic signatures (VAPS) for these four cancers, and discerned differences in the interplay between VAPS and dominant bacteria in tissues among patients with varying survival risks. Notably, clinically relevant analyses revealed prognostic capacities of the VAPS in these four cancers. Taken together, our study provides novel insights into the role of viruses in tissue in the prognosis of multiple cancers and offers guidance on the use of tissue viruses to stratify prognosis for patients with cancer.Copyright © 2023 Elsevier Ltd. All rights reserved.