HEp-2细胞系首先被鉴定为喉癌细胞。据报道，它由 HeLa 细胞系污染衍生的宫颈腺癌细胞组成。液体安息香是一种由金缕梅（金缕梅科）受伤的船体提供的渗出物，已用于治疗皮肤问题、消化性溃疡和寄生虫感染或用作防腐剂。在我们的研究中，我们旨在研究安息香对 HEp-2 癌细胞系的细胞毒性和凋亡作用。安息香（从树木中获得的土耳其香胶）的 IC50 剂量由 (3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物)测定，通过评估细胞凋亡相关基因(Bax、Bad、Bak1、p53、Bcl)的表达来确定苏合香对HEp-2癌细胞的凋亡作用。 -2、Bcl-XL、Apaf-1、Caspase2、Caspase3a、Caspase9 和 Caspase12) 通过实时定量聚合酶链式反应。发现安息香 48 小时的 IC50 值为 125 μg/mL。结果显示，苏合香减少了HEp-2喉癌细胞的数量，并增加了凋亡相关基因的表达。这些结果表明，安息香可以被认为是癌症治疗的一种选择。研究结果表明，对安息香对癌细胞的作用进行更多合格的研究会更有用。

The HEp-2 cell line was first identified as laryngeal cancer cells. Then, it was reported to consist of cervical adenocarcinoma cells derived via HeLa cell line contamination. Styrax liquidus is an exudate that is provided by the injured hull of the Liquidambar orientalis Miller (Hamamelidaceae), which has been used for the treatment of skin problems, peptic ulcers, and parasitic infections or as an antiseptic. In our study, we purposed to research the cytotoxic and apoptotic effect of styrax liquidus on HEp-2 cancer cell line.The IC50 dosage of styrax liquidus (Turkish sweet gum obtained from trees) was set by the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and the apoptotic effect of styrax liquidus on HEp-2 cancer cell was determined by assessing the expression of genes involved in apoptosis (Bax, Bad, Bak1, p53, Bcl-2, Bcl-XL, Apaf-1, Caspase2, Caspase3a, Caspase9, and Caspase12) by quantitative real-time polymerase chain reaction.The IC50 value of styrax liquidus was found to be 125 μg/mL for 48 hours. According to the results, styrax liquidus reduced the population of HEp-2 laryngeal cancer cells and increased the expression of genes which were apoptosis related. These results indicate that styrax liquidus can be thought as a choice of cancer therapy.The finding of the study showed that it would be more useful to perform more qualified studies about the effect of styrax liquidus on cancer cells.