7,8-二羟基黄酮是一种酪氨酸激酶受体激动剂，是一种黄酮类化合物，最近因其抗癌特性而引起了研究人员的关注。然而，7,8-二羟基黄酮治疗肝癌的分子途径尚不确定。我们的目的是确定7,8-二羟基黄酮对人肝癌的影响。使用人肝癌细胞系7细胞作为人肝癌细胞，并将7,8-二羟基黄酮以不同剂量应用于细胞。使用阿尔玛蓝和流式细胞术测定 7,8-二羟基黄酮的细胞毒性和细胞凋亡作用。分别通过定量实时聚合酶链反应和蛋白质印迹分析确定 7,8-二羟基黄酮对 Bcl-2、Bax、cleaved-caspase-3 基因相关 mRNA 表达和蛋白质表达的特性。7,8 -二羟基黄酮通过过表达 cleaved-caspase-3 (P=.003) 和降低 Bcl-2 (P=.038) 蛋白水平，增强人肝癌细胞系 7 中的细胞死亡。此外，cleavedcaspase-3 mRNA 过表达（P=.001）显着导致 7,8-二羟基黄酮诱导的细胞凋亡。7,8-二羟基黄酮可以通过调节 caspase 途径和抑制抗凋亡蛋白来促进凋亡细胞死亡。这些特性可能有助于7,8-二羟基黄酮预防和治疗肝癌的临床实践。

7,8-Dihydroxyflavone, a tyrosine kinase receptor agonist, is a flavonoid that has recently gained the attention of researchers due to its anticancer properties. Nevertheless, molecular pathways of 7,8-dihydroxyflavone for hepatocarcinoma are uncertain. Our aim was to identify the impact of 7,8-dihydroxyflavone on human hepatocarcinoma.Human hepatocarcinoma cell line-7 cells were used as human hepatocarcinoma cells, and 7,8-dihydroxyflavone was applied to the cells at various doses. The cytotoxic and apoptotic effects of 7,8-dihydroxyflavone were determined with Alamar Blue and flow cytometry. The properties of 7,8-dihydroxyflavone on the mRNA expressions related with Bcl-2, Bax, cleaved-caspase-3 genes, and protein expressions were determined via quantitative real-time polymerase chain reaction and western blot analysis, respectively.7,8-Dihydroxyflavone-enhanced cell death in human hepatocarcinoma cell line-7 via the overexpression of cleaved-caspase-3 (P=.003) and decreased Bcl-2 (P=.038) protein levels. Furthermore, cleavedcaspase-3 mRNA overexpression (P=.001) markedly led to 7,8-dihydroxyflavone-induced apoptosis.7,8-Dihydroxyflavone could promote apoptotic cell death by modulating caspase pathways and suppressing antiapoptotic protein. These characteristics may mediate to clinical practice of 7,8-dihydroxyflavone for prevention and therapy of hepatocarcinoma.