随着特高压直流输电技术的快速发展，特高压直流输电线路周围环境的电场强度显着增加，引发了公众对电场潜在健康影响的担忧。先前的研究表明，电磁场暴露与癌症有关。 B淋巴细胞可以通过增殖产生自身抗体和肿瘤生长因子，从而促进癌症的发展。因此，本研究探讨直流传输线产生的静电场（SEF）对B淋巴细胞增殖水平的影响及机制。雄性小鼠暴露于SEF。暴露7天和14天后，分别测量小鼠脾脏中B淋巴细胞的增殖水平。为了验证动物实验中发现的生物学效应并从信号通路的角度阐明其作用机制，将淋巴细胞暴露于SEF。暴露24、48或72小时后，测定B淋巴细胞的增殖水平、关键蛋白的表达水平和细胞周期。本研究发现SEF暴露通过刺激ERK1/2通路激活NF-κB通路，促进B淋巴细胞从G0/G1期进入S期。同时，SEF暴露也促进B淋巴细胞进入G2期。即，SEF暴露显着促进B淋巴细胞的增殖。这一发现为预防或应用 SEF 暴露引起的负面或正面影响提供了理论和实践支持，并为未来的研究提供了方向。版权所有 © 2023 Elsevier B.V. 保留所有权利。

With the rapid development of ultra-high voltage direct current (UHV DC) transmission technology, the intensity of electric fields in the surrounding environment of UHV DC transmission lines significantly increased, which raised public concerns about the potential health effects of electric fields. Previous studies have shown that the exposure of electromagnetic field was associated with cancer. B lymphocytes can produce autoantibodies and tumor growth factors through proliferation, which contributes to the development of cancer. Therefore, this study explored the effect and mechanism of static electric field (SEF) generated by DC transmission lines on the proliferation levels of B lymphocytes. Male mice were exposed to SEF. After the exposure of 7 and 14 days, the proliferation levels of B lymphocytes in the spleens of mice were measured, respectively. To validate biological effect discovered in animal experiments and elucidate the mechanism of the effect from the perspective of signaling pathways, lymphocytes were exposed to SEF. After the exposure of 24, 48 or 72 h, the proliferation levels of B lymphocytes, the expression levels of key proteins and cell cycle were determined. This study found that SEF exposure activated NF-κB pathway by stimulating ERK1/2 pathway and promoted B lymphocytes to enter S phase from G0/G1 phase. Meanwhile, SEF exposure also promoted B lymphocytes to enter G2 phase. Namely, SEF exposure significantly promoted the proliferation of B lymphocytes. This discovery provided theoretical and practical support for the prevention or application of negative or positive effects caused by SEF exposure and provided directions for future research.Copyright © 2023 Elsevier B.V. All rights reserved.