由于越来越多地采用胸部计算机断层扫描（CT）尤其是低剂量CT进行肺癌筛查，因此检测到越来越多的周围性肺部病变（PPL）需要进行组织验证以建立明确的诊断以进一步进行个体化治疗。然而，PPL 的形态学诊断仍然具有挑战性。与传统的经支气管钳活检 (TBFB) 相比，经支气管肺冷冻活检 (TBLC) 可以获取更大的样本，细胞结构保存更完整，挤压伪影更少，作为一种诊断 PPL 的新兴技术，已被证明有可能解决临床问题。解决了当前可用采样设备（例如镊子、针和刷子）的困境，并成为 PPL 的诊断基石。值得注意的是，随着 1.1mm 冷冻探头的推出，它将与先进的支气管镜导航技术更加兼容，例如径向支气管内超声 (r-EBUS)、虚拟支气管镜导航 (VBN) 和电磁导航支气管镜 (ENB)，使用TBLC 有望在 PPL 诊断中得到更多应用。虽然使用 TBLC 技术诊断 PPL 的探索还有很多工作要做，但可以预见的是，具有更大数据积累的其他研究的出现将有望丰富该领域的证据。版权所有 © 2023 Sociedade Portuguesa de Pneumologia。由 Elsevier España 出版，S.L.U.版权所有。

An increasing number of peripheral pulmonary lesions (PPLs) requiring tissue verification to establish a definite diagnosis for further individualized management are detected due to the growing adoption of lung cancer screening by chest computed tomography (CT), especially low-dose CT. However, the morphological diagnosis of PPLs remains challenging. Transbronchial lung cryobiopsy (TBLC) that can retrieve larger specimens with more preserved cellular architecture and fewer crush artifacts in comparison with conventional transbronchial forceps biopsy (TBFB), as an emerging technology for diagnosing PPLs, has been demonstrated to have the potential to resolve the clinical dilemma pertaining to currently available sampling devices (e.g., forceps, needle and brush) and become a diagnostic cornerstone for PPLs. Of note, with the introduction of the 1.1 mm cryoprobe that will be more compatible with advanced bronchoscopic navigation techniques, such as radial endobronchial ultrasound (r-EBUS), virtual bronchoscopic navigation (VBN) and electromagnetic navigation bronchoscopy (ENB), the use of TBLC is expected to gain more popularity in the diagnosis of PPLs. While much remains for exploration using the TBLC technique for diagnosing PPLs, it can be envisaged that the emergence of additional studies with larger data accrual will hopefully add to the body of evidence in this field.Copyright © 2023 Sociedade Portuguesa de Pneumologia. Published by Elsevier España, S.L.U. All rights reserved.