类器官是体外培养的三维结构，可以概括体内器官或组织的微环境、复杂结构和细胞功能。近几十年来，肝脏类器官得到了迅速发展，其在药物筛选、疾病建模和再生医学等生物医学领域的应用得到了广泛认可。然而，缺乏重复性和一致性，包括缺乏标准化的培养条件，一直是肝类器官开发和临床应用的主要障碍。研究人员确定合适的培养基成分方案非常耗时，并且某些成分的使用仍然存在争议。在这篇综述中，我们总结并比较了近年来发表的不同的肝类器官培养方法，重点关注有争议的培养基成分，并讨论了它们的优缺点。我们的目的是为肝类器官培养的发展和标准化提供有效的参考。© 2023。作者。

Organoids, three-dimensional structures cultured in vitro, can recapitulate the microenvironment, complex architecture, and cellular functions of in vivo organs or tissues. In recent decades, liver organoids have been developed rapidly, and their applications in biomedicine, such as drug screening, disease modeling, and regenerative medicine, have been widely recognized. However, the lack of repeatability and consistency, including the lack of standardized culture conditions, has been a major obstacle to the development and clinical application of liver organoids. It is time-consuming for researchers to identify an appropriate medium component scheme, and the usage of some ingredients remains controversial. In this review, we summarized and compared different methods for liver organoid cultivation that have been published in recent years, focusing on controversial medium components and discussing their advantages and drawbacks. We aimed to provide an effective reference for the development and standardization of liver organoid cultivation.© 2023. The Author(s).