智能水凝胶在癌症治疗领域继续遇到巨大的障碍。人们已经为不同的目的设计了各种各样的水凝胶材料，但仍然迫切需要具有令人满意的治疗效果的材料。在这里，我们通过物理交联的方式制备了可注射的水凝胶。碳纳米颗粒悬浮注射液（CNSI）是一种广泛应用于临床的前哨淋巴结显像剂，将β-甘油磷酸钠（β-GP）添加到温度敏感的壳聚糖（CS）水凝胶（CS/GP@ CN）作为光热疗法（PTT）的试剂。在评估了水凝胶的流变学、形态学和结构特性后，我们使用 4T1 小鼠乳腺癌细胞和 B16 黑色素瘤细胞来评估其体外特性。然后，我们将水凝胶瘤内注射到BALB/c荷瘤小鼠体内，评估体内PTT效果、抗肿瘤免疫反应和肺转移数量。令人惊讶的是，这种名为CS/GP@CN水凝胶的纳米碳水凝胶不仅具有良好的生物相容性在808nm激光照射下具有良好的PTT效应，同时还促进树突状细胞的成熟，刺激抗肿瘤免疫反应，并对肺部具有非凡的抗转移作用。总体而言，这种创新的温度敏感纳米碳水凝胶，在室温下，37°C 转化为凝胶，是一种出色的局部递送平台，具有巨大的 PTT 潜力和广阔的临床应用前景。© 2023 Tan 等人。

Intelligent hydrogels continue to encounter formidable obstacles in the field of cancer treatment. A wide variety of hydrogel materials have been designed for diverse purposes, but materials with satisfactory therapeutic effects are still urgently needed.Here, we prepared an injectable hydrogel by means of physical crosslinking. Carbon nanoparticle suspension injection (CNSI), a sentinel lymph node imaging agent that has been widely used in the clinic, with sodium β-glycerophosphate (β-GP) were added to a temperature-sensitive chitosan (CS) hydrogel (CS/GP@CN) as an agent for photothermal therapy (PTT). After evaluating the rheological, morphological, and structural properties of the hydrogel, we used 4T1 mouse breast cancer cells and B16 melanoma cells to assess its in vitro properties. Then, we intratumorally injected the hydrogel into BALB/c tumor-bearing mice to assess the in vivo PTT effect, antitumor immune response and the number of lung metastases.Surprisingly, this nanocarbon hydrogel called CS/GP@CN hydrogel not only had good biocompatibility and a great PTT effect under 808nm laser irradiation but also facilitated the maturation of dendritic cells to stimulate the antitumor immune response and had an extraordinary antimetastatic effect in the lungs.Overall, this innovative temperature-sensitive nanocarbon hydrogel, which exists in a liquid state at room temperature and transforms to a gel at 37 °C, is an outstanding local delivery platform with tremendous PTT potential and broad clinical application prospects.© 2023 Tan et al.