卵巢癌（OV）是发病率最高的妇科肿瘤，发病机制复杂。高级别浆液性卵巢囊腺癌（HGSOC）是 OV 中最流行、最恶性的亚型。 I 型角蛋白细胞骨架 19 (KRT19) 是一种中间丝蛋白，在上皮细胞的维持中发挥重要作用。然而，它在 OV 中的作用仍然很大程度上未知。本研究使用各种数据库进行了生物信息分析。具体而言，使用癌症基因组图谱 (TCGA)、基因型组织表达 (GTEx)、基因表达综合 (GEO) 和人类蛋白质图谱 (HPA) 等数据库评估 KRT19 表达。 GO-KEGG 和 GSEA 分析由 R 包进行。根据CancerSEA数据库的单细胞测序信息分析KRT19的生物学功能。通过TISIDB数据库预测KRT19表达与免疫调节剂和趋化因子的关联。与正常对照相比，卵巢样本中KRT19的表达显着上调。 KRT19 表达与 OV 预后呈负相关，进一步分析表明 KRT19 在区分 OV 癌症与正常样本方面具有良好的诊断意义。 GO-KEGG和GSEA分析表明KRT19与多种生物学功能和途径相关，包括表皮发育、顶端连接、炎症反应和上皮间质转化。通过使用不同的GEO系列，我们发现KRT19在OV相关组织中存在差异表达。此外，KRT19表达增加与OV中大多数免疫细胞的免疫浸润水平呈正相关。这项研究表明，KRT19是一种有前途的预后和诊断生物标志物，决定癌症进展，并与OV中肿瘤免疫细胞浸润相关，提示成为免疫疗法的分子靶标。© 2023 Sun 等人。

Ovarian cancer (OV) is the highest prevalent gynecologic tumor with complicated pathogenesis; high-grade serous ovarian cystadenocarcinoma (HGSOC) is the most epidemiological and malignant subtype of OV. Keratin type I cytoskeleton 19 (KRT19) is an intermediate filament protein which plays essential roles in the maintenance of epithelial cells. However, its role in OV remains largely unknown.Bioinformatic analysis with various databases was conducted in this study. In details, KRT19 expression was assessed using databases including The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), Gene Expression Omnibus (GEO) and Human Protein Atlas (HPA). GO-KEGG and GSEA analysis were performed by R packages. The biological function of KRT19 was analyzed based on the single-cell sequencing information from CancerSEA database. The association of KRT19 expression with immunomodulator and chemokine was predicted via the TISIDB database.The expression of KRT19 was significantly upregulated in ovarian samples compared with normal controls. KRT19 expression was negatively associated with prognosis in OV, and further analysis revealed that KRT19 had promising diagnostic significance in distinguishing OV cancer from normal samples. GO-KEGG and GSEA analysis indicated that KRT19 was associated with multiple biological functions and pathways including epidermis development, apical junction, inflammatory response, and epithelial mesenchymal transition. By using different GEO series, we found that KRT19 was differentially expressed in OV-associated tissues. Furthermore, the increased KRT19 expression was positively correlated with the immune infiltration levels of the most immune cells in OV.This study demonstrated that KRT19 is a promising prognosis and diagnosis biomarker that determines cancer progression and is correlated with tumor immune cells infiltration in OV, suggesting being a molecular target for immunotherapies.© 2023 Sun et al.