本研究的目的是基于生物信息学研究脑膜癌的基因表达谱和诊断应用。我们使用基因表达综合数据库（GEO），基于正常脑膜和脑膜瘤的表达数据（包含51个样本）获得了GSE43290数据集分为两组（47 个脑膜瘤样本和 4 个正常脑膜样本）。我们使用 GEO2R 工具通过将 log2 倍数变化设置为大于 2 且调整后的 p 值低于 0.05 来识别差异表达基因 (DEG)。我们使用注释、可视化和集成发现数据库（DAVID）来执行 DEG 的基因本体论、生物途径和功能注释。使用相互作用基因数据库检索工具（STRING）获得蛋白质-蛋白质相互作用（PPI）和基于马尔可夫聚类算法的模块化网络。我们的研究鉴定了358个显着DEG，其中343个是下调基因，15个是下调基因。上调。五个重要的枢纽基因（CXCL8、AGT、CXCR4、CXCL12 和 CXCL2）与各种生物学途径、分子功能和京都基因和基因组百科全书 (KEGG) 途径相关。 DEGs富含趋化因子介导的信号传导、白细胞趋化性的正向调节、第二信使介导的信号传导、正向趋化性的诱导、CXCR趋化因子受体结合和细胞因子活性的生物学途径。这些枢纽基因和途径可以作为临床研究的目标发现脑膜癌的新疗法。

The aim of this study was to research gene expression profiles and diagnostic applications of meningeal carcinoma based on bioinformatics.We used the Gene Expression Omnibus (GEO) database to obtain the GSE43290 dataset based on the expression data of normal meninges and meningiomas consisting of 51 samples divided into two groups (47 samples of meningioma tumors and four samples of normal meninges). We used the GEO2R tool to identify differentially expressed genes (DEGs) by setting the log2 fold change as greater than two and an adjusted p-value lower than 0.05. We used the database for annotation, visualization and integrated discovery (DAVID) to perform gene ontology, biological pathways and functional annotation of the DEGs. A search Tool for the Retrieval of Interacting Gene database (STRING) was used to obtain Protein-Protein Interaction (PPI) and modular networks based on the Markov clustering algorithm.Our study identified 358 significant DEGs, of which 343 were downregulated genes while 15 were upregulated. Five significant hub genes (CXCL8, AGT, CXCR4, CXCL12 and CXCL2) were associated with various biological pathways, molecular functions and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The DEGs were enriched in biological pathways of chemokine-mediated signaling, positive regulation of leukocyte chemotaxis, second messenger-mediated signaling, induction of positive chemotaxis, CXCR chemokine receptor binding and activities of cytokines.These hub genes and pathways could be targeted in clinical research to discover new treatments for meningeal carcinoma.