目的：Visnagin (Vis) 是一种在 Ammi visnaga 植物的花朵和种子中发现的化合物，具有良好的抗氧化和抗炎特性。我们的目的是研究 visnagin 对大鼠的剂量依赖性性腺保护作用，同时考虑氧化应激、细胞凋亡和炎症相关蛋白表达水平。材料和方法：28只成年大鼠分为四组，每组七只；控制、缺血/再灌注 (I/R)、I/R 30Vis 和 I/R 60Vis。对照动物不接受手术并在实验结束时处死。 I/R、I/R Vis30 和 I/R Vis60 组的大鼠暴露于睾丸缺血，I/R Vis30 和 I/R Vis60 组的动物腹膜内接受 30 或 60 mg/kg visnagin。实验结束时，睾丸组织用于测量氧化应激、细胞凋亡和炎症。结果：我们的显微镜检查表明，I/R 导致睾丸变性和形态改变，而在维斯那金治疗的动物中，这些改变得到了改善。生化分析表明，与对照组相比，I/R 组的氧化应激显着增加（p<0.05）。免疫组织化学检查显示，I/I 组中促凋亡 Bax 和 Caspase 3 表达以及促炎性肿瘤坏死因子-α (TNF-α) 水平显着上调 (p<0.05)，但增殖核抗原 (PCNA) 水平显着上调。与对照组相比，R 组显着下调（p<0.001）。结论：导致睾丸扭转的缺血是一个影响生殖健康的问题，由于活性氧（ROS）的积累，目前的手术治疗方法可能不足以恢复睾丸。我们的观察表明，visnagin 是一种潜在的联合疗法，可与睾丸缺血恢复的手术干预相结合，但我们认为需要更详细的研究来探索潜在的信号通路和 visnagin 对抗睾丸缺血再灌注损伤的强度.https://www.europeanreview.org/wp/wp-content/uploads/GRAB.jpg。

OBJECTIVE: Visnagin (Vis) is a compound found in the flowers and seeds of the Ammi visnaga plant with promising antioxidant and anti-inflammatory properties. We aimed to investigate the dose-dependent gonadoprotective effects of visnagin in rats while considering oxidative stress, apoptosis, and inflammation-related protein expression levels. MATERIALS AND METHODS: Twenty-eight adult rats were divided into four groups of seven animals each; control, ischemia/reperfusion (I/R), I/R+30Vis, and I/R+60Vis. Animals in control received no surgical application and were sacrificed at the end of the experiment. The rats in I/R, I/R + Vis30, and I/R + Vis60 were exposed to testicular ischemia and the animals in I/R + Vis30, and I/R + Vis60 groups received either 30 or 60 mg/kg visnagin intraperitoneal. At the end of the experiment, testis tissues were used for the measurement of oxidative stress, apoptosis, and inflammation. RESULTS: Our microscopic examinations indicated that I/R resulted in testicular degenerations and morphological alterations, which were improved in visnagin-treated animals. The biochemical analyses demonstrated that oxidative stress in the I/R group increased significantly (p<0.05) compared to the control group. The immunohistochemical examinations showed that pro-apoptotic Bax and Caspase 3 expressions, and pro-inflammatory tumor necrosis factor-alpha (TNF-α) levels were significantly up-regulated (p<0.05) but proliferating nuclear antigen (PCNA) levels in I/R group was significantly (p<0.001) down-regulated compared to the control group. CONCLUSIONS: Ischemia leading to testicular torsion is a reproductive health-affecting problem, and current surgical treatment methods might be insufficient to recover the testis due to the accumulation of reactive oxygen species (ROS). Our observations indicate that visnagin is a potential co-modality along with the surgical interventions for the recovery of ischemia encountered testis, but we believe the requirement of more detailed studies to explore the underlying signaling pathways and the strength of visnagin against testicular ischemia-reperfusion injury.https://www.europeanreview.org/wp/wp-content/uploads/GRAB.jpg.