大多数胃癌患者确诊时已属晚期，预后较差。因此，对于疾病检测具有高诊断价值的新型生化标记物非常重要。 Dickkopf1 (DKK1) 和细胞骨架相关蛋白 4 (CKAP4) 作为癌症患者的生物标志物已被广泛研究。在这项前瞻性研究中，使用 ELISA 试剂盒检测了 58 名胃癌患者和 41 名健康对照者的血清 DKK1 和 CKAP4 水平。根据病理TNM分期和组织学分级将患者细分为几组。术前、术后10天和30天检测胃癌患者血清中这两种蛋白的水平。与健康对照相比，胃癌组血清DKK1和CKAP4水平显着升高（p<0.05）。两种蛋白质的血清水平随着胃癌的病理阶段和组织学分级而增加。手术切除后血清CKAP4和DKK1水平下降。与第 10 天相比，术后第 30 天这两种血清水平也显着下降 (p<0.05)。胃癌组血清癌胚抗原（CEA）和糖类抗原19-9（CA19-9）阳性率均低于20%，而血清CKAP-4和DKK1的诊断价值（敏感性和特异性）超过80%。 CKAP4是具有高诊断价值的生物标志物，可用于诊断和预测胃癌的严重程度。这些蛋白质还可用于手术切除后的疾病监测。这些蛋白的诊断价值高于临床实践中常规使用的CEA和CA19-9等生物标志物。

Gastric cancer is diagnosed at an advanced stage in most patients, and the prognosis is poor. Novel biochemical markers of high diagnostic value for the detection of the disease are therefore important. Dickkopf1 (DKK1) and cytoskeleton-associated protein 4 (CKAP4) have been extensively studied as biomarkers in cancer patients.Serum DKK1 and CKAP4 levels in 58 patients with gastric cancer and 41 healthy controls were examined using an ELISA kit in this prospective study. The patients were subdivided into groups based on pathological TNM staging and histological grades. Serum levels of both proteins in the patients with gastric cancer were measured preoperatively, 10 and 30 days after surgery.Serum DKK1 and CKAP4 levels were significantly higher in the gastric cancer group compared to the healthy controls (p<0.05). Serum levels of both proteins increased in line with the pathological stage and histological grade of the gastric cancer. Serum CKAP4 and DKK1 levels decreased after surgical resection. Both serum levels also decreased significantly on day 30 after surgery compared to day 10 (p<0.05). Serum carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9) positivity rates were below 20% in the gastric cancer group, while the diagnostic value (sensitivity and specificity) of serum CKAP-4 and DKK1 exceeded 80%.DKK1 and CKAP4 are biomarkers of high diagnostic value that can be used to diagnose and predict the severity of gastric cancer. These proteins can also be employed for disease monitoring after surgical resection. The diagnostic value of these proteins is higher than that of biomarkers such as CEA and CA19-9, which are routinely used in clinical practice.