GO是一种二维纳米材料，近年来在化工、电子或医药等许多行业引起了关注。由于其独特的性质，如强度、亲水性和大比表面积以及功能化的可能性，GO 是生物医学中特别有吸引力的材料，可作为靶向药物输送的候选材料。在这种情况下，我们需要有关氧化石墨烯是否渗透到细胞中以及我们是否能够在治疗期间和治疗后检测和监测这些细胞中的GO的信息，以评估GO可能的降解过程及其在细胞室中的相互作用。这项工作介绍了拉曼光谱作为无标记检测方法，展示了将拉曼光谱与 MCR（多元曲线分辨率）分析相结合进行宫颈癌 (HeLa) 细胞中 GO 高级检测的优势。我们合成的 GO 首先通过 AFM、SEM 和拉曼光谱进行表征，然后使用 MCR-拉曼光谱检测单个 HeLa 细胞中内化的 GO。此外，通过使用我们的方法，可以研究 GO 在细胞内的分布及其长达六个月的化学稳定性，而无需使用任何额外的标记或追踪 GO。因此，MCR-拉曼光谱可能成为石墨烯纳米治疗学临床前和临床应用的新分析工具。

GO is a 2D nanomaterial that has attracted attention in many industries in recent years, such as the chemical industry, electronics or medicine. Due to its unique properties such as strength, hydrophilicity and large specific surface area with the possibility of functionalization, GO is a particularly attractive material in biomedicine as a candidate for use in targeted drug delivery. In such a case, we need information on whether graphene oxide penetrates into cells and whether we are able to detect and monitor GO in these cells during and also after the treatment to evaluate possible degradation process of GO and its interaction within the cell compartements. This work introduces the Raman spectroscopy as label-free detection method showing the advantages of combining Raman spectroscopy with MCR (Multivariate Curve Resolution) analysis for advanced detection of GO in cervical cancer (HeLa) cells. Our synthesized GO is characterized firstly by AFM, SEM and Raman spectroscopy and then MCR-Raman spectroscopy is used to detect internalized GO in individual HeLa cells. Moreover, by using our methodology, distribution of GO as well as its chemical stability inside the cell for up to six months is investigated without using any additional labeling or tracing the GO. Thus, MCR-Raman spectroscopy may become a new analytical tool in preclinical and clinical applications of graphene-based nanotheranostics.