皮肤鳞状细胞癌（CSCC）是最常见的皮肤恶性肿瘤之一，主要发生于老年人群。 CSCC 是人类第二常见的非黑色素瘤皮肤恶性肿瘤。皮肤鳞状细胞癌的发生与环境因素密切相关。微塑料作为一种新污染物，目前正在深入研究其潜在的健康影响。然而，微塑料对皮肤癌的影响尚不清楚，这是一个需要解决的重要科学问题。为此，本研究利用两种皮肤鳞状细胞癌细胞系（SCL-1和A431）研究微塑料对皮肤癌的影响，细胞行为实验表明微塑料内化到皮肤鳞状细胞癌中细胞系以时间和剂量依赖性方式。进一步的实验通过MTT、流式细胞仪、激光共聚焦显微镜、Western blotting等实验技术表明微塑料促进了皮肤癌细胞的增殖。机理研究表明，微塑料可以导致皮肤癌细胞中线粒体ROS增加，进而引起线粒体膜电位的变化，从而打开mPTP，进而导致mt-DNA从线粒体释放到细胞质中，从而激活NLRP3最终导致皮肤癌细胞增殖。我们进一步在正常皮肤细胞模型中评估了微塑料对 HaCaT 细胞的影响，结果表明微塑料通过 NLRP3 介导的炎症和烧焦死亡对正常皮肤细胞造成损伤。目前的研究表明，微塑料作为一种新污染物，可能会促进肿瘤细胞增殖，同时对正常皮肤造成损害。版权所有 © 2023 作者。由爱思唯尔公司出版。保留所有权利。

Cutaneous squamous cell carcinoma (CSCC) is one of the most common malignant tumors of the skin, occurring primarily in the elderly population. CSCC is the second most common nonmelanoma skin malignancy in humans. The development of cutaneous squamous cell carcinoma is closely linked to environmental factors. Microplastics, as a new pollutant, are currently being intensively studied for their potential health effects. However, the effect of microplastics on skin cancer is not yet known and is an important scientific question that needs to be addressed. To this end, in the current study, two skin squamous cell carcinoma cell lines (SCL-1 and A431) were utilized to investigate the effects of microplastics on skin cancer, and cell behavior experiments showed that microplastics were internalized into the skin squamous cell carcinoma cell line in a time- and dose-dependent manner. Further experiments showed that microplastics promoted the proliferation of skin cancer cells by MTT, flow cytometry, laser confocal microscopy, Western blotting and other experimental techniques. Mechanistic studies showed that microplastics could lead to increased mitochondrial ROS in skin cancer cells, which in turn caused a change in mitochondrial membrane potential, thus opening mPTP, which in turn caused the release of mt-DNA from mitochondria into the cytoplasm, thus activating NLRP3 and ultimately causing skin cancer cell proliferation. We further evaluated the effect of microplastics on HaCaT cells in a normal skin cell model and showed that microplastics caused damage to normal skin cells through NLRP3-mediated inflammation and scorch death. The current study suggests that microplastics, as a new contaminant, may promote tumor cell proliferation while causing damage to normal skin.Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.