由真菌多糖绿色合成的纳米银（AgNPs）由于其独特的特性和在众多领域的特殊应用而受到越来越多的关注。本研究通过单因素实验和响应面法建立并优化了一种经济高效、环境友好的无毒化学物质参与的Phlebopus portentosus (PPP)子实体多糖生物合成AgNPs方法。确定多糖-AgNPs(PPP-AgNPs)的最佳合成条件为反应时间140min、反应温度94℃、PPP:AgNO3比例1:11.5。 PPP-AgNPs 的形成通过目视检测颜色从淡黄到黄棕色的变化来指示。通过不同的方法对 PPP-AgNPs 进行了表征，并进一步评估了其生物活性。紫外可见（UV-Vis.）光谱在420nm处显示出尖锐的吸收峰，证实了AgNPs的形成。傅里叶变换红外 (FTIR) 分析检测到各种官能团的存在。通过 X 射线衍射 (XRD) 和分析证实了 PPP-AgNPs 的晶格指数为 (111)、(200)、(220) 和 (331)，表明 PPP-AgNPs 的 Ag 晶体结构为面心立方结构。通过高分辨率透射电子显微镜（HRTEM）发现这些颗粒是球形的。能量色散 X 射线光谱 (EDS) 测定了 PPP-AgNP 中银的存在。 PPP-AgNP 的元素相对组成百分比确定为银 (Ag) 82.5% 和氧 (O) 17.5%，并且没有表现出任何氮峰。通过BET分析计算出PPP-AgNPs的比表面积为0.5750m2/g，平均孔径为24.33nm。 Zeta电位为-4.32mV，证实了稳定性，并且通过动态光散射(DLS)计算出平均粒径为64.5nm。 PPP-AgNPs 对 DPPH 表现出显着的自由基清除活性，IC50 值为 0.1082mg/mL。 PPP-AgNP 对大肠杆菌、金黄色葡萄球菌、白色念珠菌、光滑念珠菌和近光滑念珠菌的 MIC 值为 0.05mg/mL。 PPP-AgNPs对α-葡萄糖苷酶抑制的IC50值为11.1mg/mL，计算得出PPP-AgNPs对HepG2和MDA-MB-231细胞系的IC50值分别为14.36±0.43μg/mL和40.05分别为±2.71μg/mL。根据评估可以得出结论，这些绿色合成、环境友好的PPP-AgNPs具有显着的抗氧化、抗菌、抗糖尿病和抗癌特性，有助于改善治疗效果，为临床应用提供新的可能性。版权所有©2023 Elsevier B.V.保留所有权利。

Silver nanoparticles (AgNPs) by green synthesis from fungi polysaccharides are attracting increasing attention owing to their distinctive features and special applications in numerous fields. In this study, a cost-effective and environmentally friendly biosynthesizing AgNPs method with no toxic chemicals involved from the fruiting body polysaccharide of Phlebopus portentosus (PPP) was established and optimized by single factor experiment and response surface methodology. The optimum synthesis conditions of polysaccharide-AgNPs (PPP-AgNPs) were identified to be the reaction time of 140 min, reaction temperature of 94 °C, and the PPP: AgNO3 ratio of 1:11.5. Formation of PPP-AgNPs was indicated by visual detection of colour change from yellowish to yellowish brown. PPP-AgNPs were characterized by different methods and further evaluated for biological activities. That the Ultraviolet-visible (UV-Vis.) spectroscopy displayed a sharp absorption peak at 420 nm confirmed the formation of AgNPs. Fourier transform infrared (FTIR) analysis detected the presence of various functional groups. The lattice indices of (111), (200), (220), and (331), which indicated a faced-centered-cubic of the Ag crystal structure of PPP-AgNPs, was confirmed by X-ray diffraction (XRD) and the particles were found to be spherical through high resolution transmission electron microscopy (HRTEM). Energy dispersive X-ray spectroscopy (EDS) determined the presence of silver in PPP-AgNPs. The percentage relative composition of elements was determined as silver (Ag) 82.5 % and oxygen (O) 17.5 % for PPP-AgNPs, and did not exhibit any nitrogen peaks. The specific surface area of PPP-AgNPs was calculated to be 0.5750 m2/g with an average pore size of 24.33 nm by BET analysis. The zeta potential was -4.32 mV, which confirmed the stability and an average particle size of 64.5 nm was calculated through dynamic light scattering (DLS). PPP-AgNPs exhibited significant free radical scavenging activity against DPPH with an IC50 value of 0.1082 mg/mL. The MIC values of PPP-AgNPs for E. coli, S. aureus, C. albicans, C. glabrata, and C. parapsilosis are 0.05 mg/mL. The IC50 value of the inhibition of PPP-AgNPs against α-glucosidase was 11.1 mg/mL, while the IC50 values of PPP-AgNPs against HepG2 and MDA-MB-231 cell lines were calculated to be 14.36 ± 0.43 μg/mL and 40.05 ± 2.71 μg/mL, respectively. According to the evaluation, it can be concluded that these green-synthesized and eco-friendly PPP-AgNPs are helpful to improve therapeutics because of significant antioxidant, antimicrobial, antidiabetic, and anticancer properties to provide new possibilities for clinic applications.Copyright © 2023 Elsevier B.V. All rights reserved.