系统研究了来自植物乳杆菌 WLPL09 的胞外多糖 (EPS-09) 在 B16F10 黑色素瘤小鼠模型中的抗肿瘤作用。结果显示，给予EPS-09（200mg/kg）能显着抑制荷瘤小鼠的肿瘤生长，抑制率为42.53%。同时，与模型组相比，高剂量EPS-09（200mg/kg）给药可增加脾指数（P=0.10），促进脾淋巴细胞在ConA和LPS刺激下增殖，增殖率为120.58 %和169.88%分别增加肿瘤组织中CD4和CD8 T细胞的数量（P<0.0001，P=0.0149），以及血清中细胞因子TNF-α、IFN-γ、IL-的含量。携带B16F10黑色素瘤的小鼠的IL-6（P<0.05）和IL-6（P=0.039）。转录水平分析显示，给予EPS-09（200mg/kg）可显着（P<0.05）上调凋亡相关基因P53、Caspase-3和Caspase-9的转录以及Bax/Bcl比值。 -2，与模型组相比，血管生成标志物Vegf和Fgf2的转录下调。此外，施用EPS-09可以增加厚壁菌门、瘤胃球菌科和毛螺菌科以及瘤胃球菌属的丰度，但减少普雷沃氏菌属、阿克曼氏菌属和颤螺菌属的丰度。综上，这些结果表明，给予EPS-09可以诱导肿瘤细胞凋亡，抑制肿瘤血管生成，提高免疫力，调节B16F10黑色素瘤荷瘤小鼠的肠道菌群组成，对黑色素瘤的抗肿瘤活性发挥积极作用。 © 2023。由 Elsevier B.V. 出版

Exopolysaccharide (EPS-09) from L. plantarum WLPL09 was systemically investigated for the antitumor effect in B16F10 melanoma bearing mice model. The results showed that administraion of EPS-09 (200 mg/kg) could sigificantly inhibit the tumor growth of melanoma bearing mice, with a inhibition rate of 42.53 %. Meanwhile, compared to the Model group, high dose of EPS-09 (200 mg/kg) administraion could increase the spleen index (P = 0.10), promote the splenic lymphocytes proliferation under the stimulation of ConA and LPS with a proliferation rate of 120.58 % and 169.88 %, respectively, enhance the amount of CD4+ and CD8+ T cells (P < 0.0001, P = 0.0149) in tumor tissue, as well as the serum content of cytokines, i.e., TNF-α, IFN-γ, IL-2 (P < 0.05) and IL-6 (P = 0.039) of B16F10 melanoma bearing mice. The transcriptional level analysis revealed that EPS-09 (200 mg/kg) administraion could sigificantly (P < 0.05) upregulate the transcription of apoptosis raleted genes, i.e., P53, Caspase-3 and Caspase-9, and the ratio of Bax/Bcl-2, downregulate the transcription of angiogenesis markers, i.e., Vegf and Fgf2 compared with Model group. Furthermore, administration of EPS-09 could increase the abundance of phylum Firmicutes, family Ruminococcaceae and Lachnospiraceae, and genus Ruminococcus, but reduce the abundance of genus Prevotella, Akkermansia and Oscillospira. Taken together, these results indicate that administration of EPS-09 can induce apoptosis of tumor cell, inhibit tumor angiogenesis, improve the immunity, regulate the intestinal microbiota composition of B16F10 melanoma bearing mice, and play positive roles in the antitumor activity against melanoma.Copyright © 2023. Published by Elsevier B.V.