多种化学药物与纳米技术已被证明是一种有效的癌症治疗技术。当联合服用时，卡巴他赛（CTX）和顺铂（PT）在几种不同癌症的化疗中比单独使用药物具有更优异的细胞毒作用。然而，在癌症患者中使用这些化疗药物会产生一些严重的副作用。金纳米材料（AuNMs）因其直径小、易于表面修饰、良好的生物相容性和强的细胞渗透性而有望作为药物载体。这项工作旨在确定 AuNM 封装的 CTX 和 PT 对人胶质瘤 U87 癌细胞的作用。 AuNM 的制造实现了负表面电荷、多分散指数和平均尺寸。当在 U87 细胞上进行测试时，CTX 和 PT 与 AuNM 结合的联合细胞毒性作用比单独使用任何一种药物的作用都要大。游离PT的半数抑制浓度(IC50)值为54.7μg/mL(24小时)和4.8gμg/mL(72小时)。 MTT 测定结果显示，细胞生长降低了时间和浓度依赖性 AuNM、游离 CTX、游离 PT 和 AuNMs@CTX/PT 诱导的细胞毒性，并最终通过细胞凋亡导致 U87 细胞死亡。生化凋亡染色技术研究细胞的形态变化（吖啶橙和溴化乙锭（AO-EB）和核染色（DAPI）技术）。 AO-EB 和核染色结果表明纳米颗粒有效杀死癌细胞。此外，流式细胞术分析检查了细胞死亡的模式。因此，AuNMs@CTX/PT 在不同癌细胞的癌症治疗中具有良好的潜力。版权所有 © 2023 P. Baby Shakila 等人。

Multiple chemodrugs with nanotechnology have proven to be an effective cancer treatment technique. When taken combined, cabazitaxel (CTX) and cisplatin (PT) have more excellent cytotoxic effects than drugs used alone in the chemotherapy of several different cancers. However, several severe side effects are associated with using these chemotherapy drugs in cancer patients. Gold nanomaterials (AuNMs) are promising as drug carriers because of their small diameter, easy surface modifications, good biocompatibility, and strong cell penetration. This work aimed to determine the CTX and PT encapsulated with AuNMs against human glioma U87 cancer cells. The fabrication of the AuNMs achieved a negative surface charge, polydispersity index, and the mean sizes. The combined cytotoxic effect of CTX and PT bound to AuNMs was greater than that of either drug alone when tested on U87 cells. The half inhibitory concentration (IC50) values for free PT were 54.7 μg/mL (at 24 h) and 4.8 g μg/mL (at 72 h). Results acquired from the MTT assay show cell growth decreases time- and concentration-dependent AuNMs, free CTX, free PT, and AuNMs@CTX/PT-induced cytotoxicity and, ultimately, the cell death of U87 cells via apoptosis. The biochemical apoptosis staining techniques investigated the cells' morphological changes of the cells (acridine orange and ethidium bromide (AO-EB) and nuclear staining (DAPI) techniques). The AO-EB and nuclear staining results reveal that the NPs effectively killed cancer cells. Furthermore, the flow cytometry analysis examined the mode of cell death. Therefore, AuNMs@CTX/PT has excellent potential in the cancer therapy of different cancer cells.Copyright © 2023 P. Baby Shakila et al.