背景：由于复杂的遗传-微环境相互作用，结直肠癌（CRC）的预后各不相同，并且强调了多种基于基因的预后模型。材料和方法：在这项工作中，开发了基于免疫相关基因表达的模型，并计算了每个样本的得分。分析模型与临床信息、免疫浸润、药物反应和生物学途径的相关性。结果：高分样本的生存期（总生存期和无进展生存期）明显长于低分样本，这在包含 1,325 个样本的 7 个数据集中进行了验证（GSE17536 (N = 115)、GSE17537 (N = 55)、GSE33113 (N = 90)、GSE37892 (N = 130)、GSE38832 (N = 74)、GSE39582 (N = 481) 和 TCGA (N = 380))。该评分与年龄和分期等临床指标显着相关，并进一步与PD-1/PD-L1基因表达相关。此外，高分样本的 APC 显着较高，MUC5B 突变率较低。高分样本显示更多的免疫浸润（包括 CD4 和 CD8 T 细胞、M1/M2 巨噬细胞和 NK 细胞）。富集通路分析表明，癌症相关通路，包括免疫相关通路，在高分样本中显着激活，并且某些药物的IC50值显着低于低分样本。结论：基于免疫相关基因开发的模型是稳健的，反映了CRC的各种状态，可能是一个潜在的临床指标。版权所有©2023 Kang，Chen，Ma，Yan和Wang。

Bakcground: Prognosis of colorectal cancer (CRC) varies due to complex genetic-microenviromental interactions, and multiple gene-based prognostic models have been highlighted. Material and Method: In this work, the immune-related genes' expression-based model was developed and the scores of each sample were calculated. The correlation between the model and clinical information, immune infiltration, drug response and biological pathways were analyzed. Results: The high-score samples have a significantly longer survival (overall survival and progression-free survival) period than those with a low score, which was validated across seven datasets containing 1,325 samples (GSE17536 (N = 115), GSE17537 (N = 55), GSE33113 (N = 90), GSE37892 (N = 130), GSE38832 (N = 74), GSE39582 (N = 481), and TCGA (N = 380)). The score is significantly associated with clinical indicators, including age and stage, and further associated with PD-1/PD-L1 gene expression. Furthermore, high-score samples have significantly higher APC and a lower MUC5B mutation rate. The high-score samples show more immune infiltration (including CD4+ and CD8+ T cells, M1/M2 macrophages, and NK cells). Enriched pathway analyses showed that cancer-related pathways, including immune-related pathways, were significantly activated in high-score samples and that some drugs have significantly lower IC50 values than those with low score. Conclusion: The model developed based on immune-related genes is robust and reflected various statuses of CRC and may be a potential clinical indicator.Copyright © 2023 Kang, Chen, Ma, Yan and Wang.