E6 和 E7 人乳头瘤病毒 (HPV) 癌蛋白分别通过靶向 p53 和 pRb 抑制肿瘤抑制途径，在受感染宫颈癌细胞的恶性转化中发挥重要作用。本研究旨在探讨 Oroxylum indicum (OI) 叶的甲醇提取物对 SiHa 宫颈癌细胞的抗癌作用。使用蛋白质印迹分析评估实验前后凋亡相关蛋白（Bcl-2、caspase (cas)-3 和 cas-9）、病毒癌蛋白（E6 和 E7）和肿瘤抑制蛋白（p53 和 pRb）的表达E6/E7 小干扰 RNA (siRNA) 转染。此外，还使用实时 (RT)-PCR 评估了 E6/E7 mRNA 表达水平。本研究表明，OI 提取物有效阻碍 SiHa 细胞的增殖，并促进 cas-3 和 cas-9 表达增加，但降低 Bcl-2 表达。 OI 提取物抑制 E6/E7 病毒癌蛋白，导致 SiHa 细胞中 p53 和 pRb 肿瘤抑制基因上调。此外，OI 提取物和棉酚黄酮类化合物的组合处理诱导 p53 和 pRb 的恢复。在 siRNA 转染细胞中使用 OI 提取物处理还进一步抑制了 E6/E7 表达水平以及 p53 和 pRb 蛋白的进一步上调。总之，OI 提取物处理 siRNA 转染的 SiHa 细胞可以附加且有效地阻止 E6 和 E7 依赖性 p53 和 pRb 降解。所有这些数据表明，可以探索 OI 在具有 HPV 整合基因组的宫颈癌细胞中的化疗潜力。© 2023。作者获得 Springer Science Business Media, LLC（Springer Nature 的一部分）的独家许可。

E6 and E7 human papillomavirus (HPV) oncoproteins play a significant role in the malignant transformation of infected cervical cancer cells via suppression of tumour suppressor pathways by targeting p53 and pRb, respectively. This study aimed to investigate the anticancer effects of Oroxylum indicum (OI) leaves' methanol extract on SiHa cervical cancer cells. Expression of apoptosis-related proteins (Bcl-2, caspase (cas)-3, and cas-9), viral oncoproteins (E6 and E7), and tumour suppressor proteins (p53 and pRb) were evaluated using western blot analysis before and after E6/E7 small interfering RNAs (siRNAs) transfection. In addition, the E6/E7 mRNA expression levels were assessed with real-time (RT)-PCR. The present study showed that the OI extract effectively hindered the proliferation of SiHa cells and instigated increments of cas-3 and cas-9 expressions but decreased the Bcl-2 expressions. The OI extract inhibited E6/E7 viral oncoproteins, leading to upregulation of p53 and pRb tumour suppressor genes in SiHa cells. Additionally, combinatorial treatment of OI extract and gossypin flavonoid induced restorations of p53 and pRb. Treatment with OI extract in siRNA-transfected cells also further suppressed E6/E7 expression levels and further upregulations of p53 and pRb proteins. In conclusion, OI extract treatment on siRNAs-transfected SiHa cells can additively and effectively block E6- and E7-dependent p53 and pRb degradations. All these data suggest that OI could be explored for its chemotherapeutic potential in cervical cancer cells with HPV-integrated genomes.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.