前列腺特异性抗原（PSA）是临床诊断中早期前列腺癌（PCa）检测的常见血清相关生物标志物。但由于PSA特异性较低，早期准确诊断PCa存在困难。在此，我们设计了一种新型溶液门控石墨烯场晶体管（SGGT）生物传感器，具有双门，用于双生物标志物检测。传感机制是固定在栅电极表面的设计适体可以捕获PSA和肌氨酸（SAR）生物分子并诱导SGGT双电层（EDL）的电容变化。 PSA 和 SAR 生物标志物的检测限 (LOD) 可以达到 0.01 fg/mL，比之前报道的检测方法低 3-4 个数量级。 PSA和SAR的检测时间分别为约4.5分钟和约13分钟，明显快于传统方法的检测时间（1-2小时）。临床血清样本检测表明，该生物传感器能够区分前列腺癌患者和对照组，诊断准确率可达100%。 SGGT生物传感器可以集成到便携式平台中，诊断结果可以直接显示在智能手机/Pad上。因此，生物传感器的集成便携式平台可以通过双生物标志物检测来区分癌症类型。本文受版权保护。保留所有权利。本文受版权保护。版权所有。

Prostate-specific antigen (PSA) is the common serum-relevant biomarker for early prostate cancer (PCa) detection in clinical diagnosis. However, it is difficult to accurately diagnose PCa in the early stage due to the low specificity of PSA. Herein, we designed a new solution-gated graphene field transistor (SGGT) biosensor with dual-gate for dual-biomarker detection. The sensing mechanism is that the designed aptamers immobilized on the surface of the gate electrodes can capture PSA and sarcosine (SAR) biomolecules and induce the capacitance changes of the electric double layers (EDLs) of SGGT. The limit of detections (LODs) of PSA and SAR biomarkers can reach 0.01 fg/mL which is 3-4 orders of magnitude lower than previously reported assays. The detection time of PSA and SAR is ≈ 4.5 and ≈13 minutes which is significantly faster than the detection time (1-2 h) of conventional methods. The clinical serum samples testing demonstrates that the biosensor can distinguish the PCa patients from the control group and the diagnosis accuracy can reach 100%. The SGGT biosensor can be integrated into the portable platform and the diagnostic results can directly display on the smartphone/Pad. Therefore, the integrated portable platform of the biosensor can distinguish cancer types through the dual-biomarker detection. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.