在本文中，研究了钒酸盐（V）与十二烷基硫酸盐对牛血清白蛋白的结合竞争对钒（V）物质对抗前列腺癌细胞的细胞毒性的影响。观察到 pH 和 SDS 依赖性钒酸盐 (V)-BSA 相互作用。在 pH5 时，只有 1 个位点能够结合 10 个钒酸盐 (V) 离子 (logK(ITC)1=4.96±0.06；ΔH(ITC)1=-1.04±0.03kcalmol-1)，而在 pH7 时，有两个位点发现了蛋白质上独特的结合位点，分别饱和了两个和七个 V(V) 离子（logK(ITC)1=6.11±0.06；ΔH(ITC)1=0.78±0.12kcalmol-1；logK(ITC)2 =4.80±0.02；ΔH(ITC)2=-4.95±0.14kcalmol-1)。 SDS 影响所得 V(V)-BSA 复合物的化学计量和稳定性。最后，在培养基中存在和不存在 SDS 的情况下检查了钒酸盐 (V) 对前列腺癌细胞（PC3 系）的细胞毒性。在用 100μM 钒酸盐 (V) 孵育 24 小时的情况下，约在 SDS 存在的情况下，观察到 PC3 细胞的活力降低了 20%。然而，在其他考虑的情况下（不同浓度和孵育时间），SDS 不会影响钒酸盐 (V) 对所研究的前列腺癌细胞的剂量依赖性作用。版权所有 © 2023。由 Elsevier B.V 出版。

In this article, the implications of binding competition of vanadates(V) with dodecyl sulfates for bovine serum albumin on cytotoxicity of vanadium(V) species against prostate cancer cells have been investigated. The pH- and SDS-dependent vanadate(V)-BSA interactions were observed. At pH 5, there is only one site capable of binding ten vanadates(V) ions (logK(ITC)1 = 4.96 ± 0.06; ΔH(ITC)1 = -1.04 ± 0.03 kcal mol-1), whereas at pH 7 two distinctive binding sites on protein were found, saturated with two and seven V(V) ions, respectively (logK(ITC)1 = 6.11 ± 0.06; ΔH(ITC)1 = 0.78 ± 0.12 kcal mol-1; logK(ITC)2 = 4.80 ± 0.02; ΔH(ITC)2 = - 4.95 ± 0.14 kcal mol-1). SDS influences the stoichiometry and the stability of the resulting V(V)-BSA complexes. Finally, the cytotoxicity of vanadates(V) against prostate cancer cells (PC3 line) was examined in the presence and absence of SDS in the culture medium. In the case of a 24-h incubation with 100 μM vanadate(V), a ca. 20 % reduction in viability of PC3 cells was observed in the presence of SDS. However, in other considered cases (various concentrations and time of incubation) SDS does not affect the dose-dependent action of vanadates(V) on the investigated prostate cancer cells.Copyright © 2023. Published by Elsevier B.V.