结直肠癌（CRC）是世界上第三大常见癌症。 miRNA-22 已成为具有诊断意义的潜在候选者；然而，其在结直肠正常-腺瘤-癌转变过程中的表达谱仍未被探索。在这项研究中，我们评估了不同阶段 CRC 患者的血清 miRNA-22 水平。研究队列由 49 名健康对照者、50 名息肉患者、51 名结直肠腺瘤 (CRA) 患者和 50 名 CRC 病例组成，均通过直肠结肠镜检查和病理活检证实。采用实时定量聚合酶链反应验证血清 miRNA-22 在 CRC 进展不同阶段之间的显着差异表达。采用2-ΔΔCT方法评估血清miRNA-22表达水平的相对变化。我们的结果显示，四组之间的血清 miR-22 表达在性别、腺瘤分级、位置或 TNM 分类阶段方面没有显着差异。值得注意的是，与对照组相比，CRC 和 CRA 组均表现出更高的 miR-22 表达水平 (p = 0.0001，p = 0.0004)，其中 CRA 和 CRC 组表现出比息肉组更高的表达水平 (p = 0.02，p = 0.043）。有序多类别逻辑回归分析模型显示，在区分个体 CRC 和健康人时，年龄、性别、吸烟状况和 miR-22 表达的利用共同表现出曲线下面积的最高值 (AUC=0.748)。在区分 CRA 和健康个体时，miR-22 表达的独立因子表现出最显着的预测能力 (AUC=0.753)。此外，miR-22 的独立表达在息肉和 CRA/CRC 之间表现出可辨别的潜在分化（AUC=0.654，0.636）。值得注意的是，在区分息肉和健康个体时，年龄因素显示出最大的区分力（AUC=0.741）。我们的研究结果为考虑将 miR-22 作为 CRC 早期筛查的潜在生物标志物提供了支持证据。尽管如此，miR-22 在结直肠病变中调节的分子机制仍需研究。版权所有 © 2023。由 Elsevier B.V. 出版。

Colorectal cancer (CRC) is the third most common cancer in the world. MiRNA-22 has emerged as a potential candidate with diagnostic significance; however, its expression profile across the normal-adenoma-carcinoma transition in colorectal remains unexplored. In this study, we evaluated serum miRNA-22 levels in patients with varying stages of CRC. The study cohort comprised 49 healthy controls, 50 patients with polyps, 51 individuals with colorectal adenoma (CRA), and 50 cases of CRC, confirmed through proctocolonoscopy and pathological biopsy. Real-time quantitative polymerase chain reaction was employed to validate the significantly differential expression of serum miRNA-22 among different stages of CRC progression. The 2-ΔΔCT method was utilized to assess the relative changes in serum miRNA-22 expression levels. Our results revealed no significant differences in gender, adenoma grade, location, or TNM classification stage in terms of serum miR-22 expression across the four groups. Notably, both the CRC and CRA groups exhibited higher miR-22 expression levels compared to the control group (p = 0.0001, p = 0.0004), with the CRA and CRC groups displaying higher expression levels than the polyp group (p = 0.02, p = 0.043). Ordered multicategorical logistic regression analysis model revealed the utilization of age, gender, smoking status, and miR-22 expression collectively exhibited the highest value for the area under the curve (AUC=0.748) in the discrimination between individuals CRC and healthy. The independent factor of expression of miR-22 demonstrated the most notable predictive capacity (AUC=0.753) when distinguishing between CRA and healthy individuals. Furthermore, the independent expression of miR-22 exhibited discernible potential (AUC=0.654, 0.636) differentiation between polyps and CRA/ CRC. Notably, the factor of age displayed the most substantial discriminatory power (AUC=0.741) when distinguishing between polyps and healthy individuals. Our findings provide supportive evidence for considering miR-22 as a potential biomarker for CRC early screening. Nonetheless, the molecular mechanisms of miR-22 regulation in colorectal lesions still need to be investigated.Copyright © 2023. Published by Elsevier B.V.