研究动态
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使用超声引导右心室注射建立小鼠肺转移模型。

Establishing a mouse model of lung metastases using ultrasound-guided right heart ventricle injection.

发表日期:2023 Nov 03
作者: Amanda Labora, Amanda Creech, Hailey Lee, Erin Tabornal, Caius Radu, Timothy Donahue
来源: Cellular & Molecular Immunology

摘要:

我们报告了一种通过在超声引导下将肿瘤细胞选择性注射到右心室来生成肺转移小鼠模型的技术。首先,我们如前所述描述细胞制备和参考动物制备。然后,我们使用先前描述的 3D 打印仪器稳定装置详细介绍该技术。最后,我们描述了使用生物发光成像的肿瘤生长监测。有关该协议的使用和执行的完整详细信息,请参阅 Labora 等人 1。版权所有 © 2023 作者。由爱思唯尔公司出版。保留所有权利。
We report a technique to generate a murine model of lung metastases by selectively injecting tumor cells into the right heart ventricle under ultrasound guidance. First, we describe cell preparation and reference animal preparation as previously described. We then detail the technique using a previously described 3D-printed instrument stabilization device. Finally, we describe tumor growth surveillance using bioluminescent imaging. For complete details on the use and execution of this protocol, please refer to Labora et al.1.Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.