从人胃粘膜中分离印戒细胞的方案。
Protocol for isolation of signet ring cells from human gastric mucosa.
发表日期：2023 Nov 03
作者：
Sarah G Samaranayake, Lauren A Gamble, Cassidy Bowden, Benjamin L Green, Amber F Gallanis, Dilara Akbulut, Niharika Shah, Jonathan M Hernandez, Jeremy L Davis
来源：
Cellular & Molecular Immunology
摘要：
超过 90% 的具有种系致病性 CDH1 变异的个体将携带隐匿性的、微观的印戒细胞癌病灶，能够进展为晚期弥漫型胃癌。在这里，我们提出了一种从人胃组织中高活力悬浮印戒细胞的方案。我们描述了胃粘膜分离和组织解离的步骤。然后，我们详细介绍了将细胞嵌入 HistoGel 进行免疫组织化学染色以及流式细胞术和单细胞测序等其他应用的程序。由 Elsevier Inc. 出版。
More than 90% of individuals with germline pathogenic CDH1 variants will harbor occult, microscopic foci of signet ring cell carcinomas capable of progressing to advanced diffuse-type gastric cancer. Here, we present a protocol for high viability suspension of signet ring cells from human gastric tissue. We describe the steps for gastric mucosa isolation and tissue dissociation. We then detail procedures for embedding cells into HistoGel for immunohistochemistry staining and additional applications such as flow cytometry and single-cell sequencing.Published by Elsevier Inc.