用于体外和体内研究的小鼠免疫效应细胞扩增方案。
Protocol for the expansion of mouse immune effector cells for in vitro and in vivo studies.
发表日期：2023 Nov 04
作者：
Thomas Look, Hanna Meister, Michael Weller, Tobias Weiss
来源：
Cellular & Molecular Immunology
摘要：
研究癌症过继性细胞疗法的临床前研究需要不同免疫效应细胞的可重复且有效的扩增。在这里，我们提供了快速扩增小鼠 T 细胞、自然杀伤 (NK) 细胞和骨髓源性巨噬细胞 (BMDM) 的方案。我们描述了 αCD3/αCD8 板涂层、分离脾细胞以及扩增 T 细胞和 NK 细胞的步骤。此外，我们详细介绍了骨髓分离和 BMDM 分化的程序。版权所有 © 2023 作者。由爱思唯尔公司出版。保留所有权利。
Reproducible and efficient expansion of different immune effector cells is required for pre-clinical studies investigating adoptive cell therapies against cancer. Here, we provide a protocol for the rapid expansion of mouse T cells, natural killer (NK) cells, and bone-marrow-derived macrophages (BMDMs). We describe steps for αCD3/αCD8 plate coating, isolating splenocytes, and expanding T cells and NK cells. Further, we detail procedures for bone marrow isolation and BMDM differentiation.Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.