结直肠癌（CRC）是一种常见的胃肠道恶性肿瘤。环状 RNA (circRNA) 的失调与 CRC 的进展相关。然而，circ_0013339 (hsa_circ_0013339) 在 CRC 中的作用仍不清楚。通过定量实时聚合酶链反应测量 CRC 中 circ_0013339、miR-136-5p 和 SRY-box 转录因子 9 (SOX9) 的水平（定量RT-PCR）。集落形成和 5-乙炔基-2'-脱氧尿苷 (EdU) 测定用于检测细胞增殖。使用细胞计数试剂盒 8 (CCK8) 测定来测量细胞活力。进行蛋白质印迹测定以检查蛋白质表达。通过双荧光素酶报告基因测定测试miR-136-5p与circ_0013339或SOX9之间的关系。通过异种移植实验检查sh-circ_0013339对体内肿瘤生长的影响。Circ_0013339在CRC组织和细胞中表达升高，circ_0013339敲低减少CRC细胞的生长。 MiR-136-5p 受 circ_0013339 调控。 MiR-136-5p 缺陷改善了 circ_0013339 沉默对 CRC 细胞恶性行为的影响。 Circ_0013339 通过 miR-136-5p 调节 SOX9 表达。添加 SOX9 可以逆转 miR-136-5p 过表达对 CRC 细胞行为的影响。此外，circ_0013339的沉默抑制了体内异种移植肿瘤的生长。Circ_0013339通过SOX9的miR-136-5p依赖性调节来调节CRC的进展，揭示了circ_0013339在CRC中的一种新的调节机制。©2023。作者，获得 Springer Science Business Media, LLC（Springer Nature 的一部分）的独家许可。

Colorectal cancer (CRC) is a common gastrointestinal malignancy. Dysregulation of circular RNAs (circRNAs) is associated with the progression of CRC. However, the role of circ_0013339 (hsa_circ_0013339) in CRC is still not clear.The levels of circ_0013339, miR-136-5p, and SRY-box transcription factor 9 (SOX9) in CRC were gauged by quantitative real-time polymerase chain reaction (qRT-PCR). Colony formation and 5-Ethynyl-2'-deoxyuridine (EdU) assays were used to detect cell proliferation. Cell counting kit-8 (CCK8) assay was used to measure cell viability. Western blot assay was performed to examine protein expression. The relationship between miR-136-5p and circ_0013339 or SOX9 was tested by dual-luciferase reporter assay. The effect of sh-circ_0013339 on tumor growth in vivo was examined by xenograft experiments.Circ_0013339 expression was elevated in CRC tissues and cells, and circ_0013339 knockdown diminished the growth of CRC cells. MiR-136-5p was regulated by circ_0013339. MiR-136-5p deficiency ameliorated the effects of circ_0013339 silencing on CRC cell malignant behaviors. Circ_0013339 modulated SOX9 expression through miR-136-5p. SOX9 addition reversed the effects of miR-136-5p overexpression on CRC cell behaviors. Moreover, silencing of circ_0013339 suppressed the growth of xenograft tumors in vivo.Circ_0013339 regulates the progression of CRC through miR-136-5p-dependent regulation of SOX9, uncovering a novel regulatory mechanism of circ_0013339 in CRC.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.