制造了一种创新的电化学生物传感器，用于灵敏检测人表皮生长因子受体 2 (HER2) 蛋白，该蛋白被认为是乳腺癌诊断和治疗评估的重要肿瘤标志物。该传感器是使用 Apt 和 PNA 作为识别探针并结合磁性 Fe3O4/α-Fe2O3@Au 纳米复合材料构建的。该传感策略旨在降低HER2的检测限，并避免大分子HER2在电极表面造成的大空间干扰。刚性结构双链DNA（Apt/ssDNA）旨在提高传感器的灵敏度。 Apt捕获大分子HER2蛋白，同时释放ssDNA链，引起电化学信号的敏感变化。 PNA捕获释放的ssDNA链，将HER2引起的电化学信号变化转化为短链ssDNA数量引起的电化学信号变化，从而扩大了检测范围。在优化条件下，该传感策略实现了HER2的超低检测LOD（4.1 fg·mL-1），检测范围为10 fg·mL-1-5 × 106 fg·mL-1。实验结果证实电化学生物传感器具有良好的选择性、重现性和储存稳定性。加标血清样品的分析显示回收率为 95.9-115.7%，这表明血清样品中 HER2 检测的前景广阔。版权所有 © 2023 Elsevier B.V. 保留所有权利。

An innovative electrochemical biosensor was fabricated for sensitive detection of human epidermal growth factor receptor 2 (HER2) protein, which was considered as an essential tumor marker for diagnosis and treatment evaluation of breast cancer. The sensor was constructed using Apt and PNA as recognition probes incorporated with magnetic Fe3O4/α-Fe2O3@Au nanocomposites. The sensing strategy was designed to lower the detection limit of HER2, and avoid the large steric interference caused by macromolecular HER2 on the electrode surface. Rigid structure dsDNA (Apt/ssDNA) was designed to improve the sensitivity of the sensor. Apt captured the macromolecular HER2 protein, and ssDNA chains were simultaneously released, causing a sensitive change in the electrochemical signal. PNA captured the released ssDNA chains, which converted the electrochemical signal changes caused by HER2 to those caused by the number of short strand ssDNA, so the detection range was extended. Under optimized conditions, this sensing strategy realized an ultra-low detection LOD of HER2 (4.1 fg·mL-1), and the detection range was 10 fg·mL-1-5 × 106 fg·mL-1. The experimental results confirmed that the electrochemical biosensor had excellent selectivity, reproducibility, and storage stability. Analysis of spiked serum samples exhibited a recovery rate of 95.9-115.7 %, which indicated great promise for HER2 detection in serum samples.Copyright © 2023 Elsevier B.V. All rights reserved.