在这项研究中，提出了一种利用硫化氢 (H2S) 和钌氮碳 (Ru-N-C) 纳米酶之间相互作用的新方法，推进了 H2S 和胱硫醚 γ-裂解酶 (CSE) 生物传感技术。利用 Ru-N-C 纳米酶固有的类过氧化物酶活性和 H2S 引起的显着抑制作用，开发了一种高效、简单且经济的测定方法。这种创新方法可以对各种来源的 H2S 进行多功能实时监测，包括专门的供体和本地细菌生产。此外，该测定已用于揭示离体肿瘤细胞和组织内的相互作用，通过改进的比色方法提供更清晰、更简单的 CSE 表达水平视图。这一贡献增强了我们对 H2S 代谢复杂作用的理解，代表了纳米酶在生物医学研究中多功能应用的重大进步。版权所有 © 2023 Elsevier B.V. 保留所有权利。

In this study, a novel approach exploiting the interactions between hydrogen sulfide (H2S) and ruthenium-nitrogen-carbon (Ru-N-C) nanozymes is presented, advancing H2S and cystathionine γ-lyase (CSE) biosensing techniques. Utilizing the intrinsic peroxidase-like activity of Ru-N-C nanozymes and the noticeable inhibition effect caused by H2S, an efficient, simple, and economical assay has been developed. This innovative method allows for the versatile real-time monitoring of H2S from various sources, including specialized donors and native bacterial production. Furthermore, the assay has been applied to reveal the interactions within tumor cells and tissues ex vivo, providing a clearer and simpler view of CSE expression levels through an improved colorimetric method. This contribution enhances our understanding of the complex roles of H2S metabolism and represents a significant advancement in the versatile application of nanozymes in biomedical research.Copyright © 2023 Elsevier B.V. All rights reserved.