选择性剪接是导致 mRNA 翻译产生不同蛋白质的过程，对于肿瘤的发展至关重要。在本研究中，我们利用生物信息学分析构建了与肝癌选择性剪接事件相关的预后模型，包括CSAD、AFMID、ZDHHC16和IRF3的选择性剪接。该模型是独立的预后因素，可以准确预测患者的预后。 IRF3是与免疫反应相关的转录因子。其选择性剪接可影响多种与预后相关的基因的表达，在肿瘤微环境中发挥重要作用。我们还在 mRNA 水平验证了 IRF3 外显子跳跃异构体在肝癌中的表达。总之，我们发现IRF3的选择性剪接对于肝癌的发展至关重要。这项研究为肝癌治疗的发展提供了新的见解。

Alternative splicing is a process causing mRNA translation to produce different proteins, and it is crucial for the development of tumours. In this study, we constructed a prognostic model related to alternative splicing events in hepatocarcinoma using bioinformatics analysis, including the alternative splicing of CSAD, AFMID, ZDHHC16, and IRF3. The model is an independent prognostic factor and can accurately predict a patient's prognosis. IRF3 is a transcription factor related to the immune response. Its alternative splicing can affect the expression of various genes related to prognosis and plays an essential role in the tumour microenvironment. We also verified the expression of IRF3 exon skipping isoform in hepatocarcinoma at the mRNA level. In conclusion, we discovered that the alternative splicing of IRF3 is essential for the development of hepatocarcinoma. This study provides new insight into the development of treatments for hepatocarcinoma.