本研究旨在确定单独的 Solanum aculeatissimum 水提取物 (SaCE) 提取物以及利用 S. aculeatissimum 水提取物 (SaCE) 绿色合成产生的银纳米粒子 (AgNP) 的生物特性。使用紫外可见分光光度法、扫描透射电子显微镜 (TEM)、能量色散光谱 (EDS)、zeta 电位 (ZP)、动态光散射 (DLS) 对这些合成的 SaCE AgNP 进行表征。测定总多酚、黄酮、皂苷含量。此外，还采用高效液相色谱-质谱法（HPLC-MS）来鉴定该提取物中的成分。抗氧化活性通过DPPH自由基清除和铁离子还原力（FRAP）方法测定。通过电泳 (RME) 中的相对迁移率和游离氨基的测定来证明抗糖化活性。还检查了对酪氨酸酶的抑制活性。进行分子对接分析以评估与 DNA 和酪氨酸酶的分子相互作用。还测量了抗肿瘤活性SaCE。 SaCE 和 AgNP 的植物化学分析表明存在多酚（1000.41 和 293.37mg 没食子酸当量/g）、黄酮类化合物（954.87 和 479.87mg 芦丁当量/g）、皂苷（37.89% 和 23.01% 总皂苷），特别是甾体皂苷（aculeatiside A）和 B）。 SaCE和AgNPs均表现出显着的抗氧化活性（在DPPH测试中分别为73.97％和56.27％，在FRAP测试中分别为874.67和837.67μM Trolox当量/g）和抗糖化活性（72.81％和67.98％游离氨基，在RME中观察到的结果）。 SaCE 和 AgNPs 对酪氨酸酶的抑制活性分别为 33.2%、36.1%。计算机模拟分析证明了甾体皂苷、DNA 或酪氨酸酶之间的相互作用。 SaCE 对多种人类肿瘤细胞表现出抗肿瘤作用。数据表明，单独提取 SaCE 和从 SaCE 合成的 AgNP 具有令人感兴趣的生物学特性，可用于新的医学治疗制剂。

The present study aimed to determine the biological properties of an extract of Solanum aculeatissimum aqueous extract (SaCE) alone as well as silver nanoparticles (AgNPs) generated by green synthesis utilizing S. aculeatissimum aqueous extract (SaCE). These synthesized SaCE AgNPs were characterized using UV-VIS spectrophotometry, scanning transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), zeta potential (ZP), dynamic light scattering (DLS). Determination of total polyphenols, flavonoids, saponins content was conducted. In addition, high performance liquid chromatography-mass spectrometry (HPLC-MS) was employed to identify constituents in this extract. Antioxidant activity was determined by DPPH radical scavenging and ferric ion reducing power (FRAP) methods. Antiglycation activity was demonstrated through relative mobility in electrophoresis (RME) and determination of free amino groups. The inhibitory activity on tyrosinase was also examined. Molecular docking analyses were performed to assess the molecular interactions with DNA and tyrosinase. The antitumor activity SaCE was also measured. Phytochemical analysis of SaCE and AgNPs showed presence polyphenols (1000.41 and 293.37 mg gallic acid equivalent/g), flavonoids (954.87 and 479.87 mg rutin equivalent/g), saponins (37.89 and 23.01% total saponins), in particular steroidal saponins (aculeatiside A and B). Both SaCE and AgNPs exhibited significant antioxidant (respectively, 73.97%, 56.27% in DPPH test, 874.67 and 837.67 μM Trolox Equivalent/g in FRAP test) and antiglycation activities (72.81 and 67.98% free amino groups, results observed in RME). SaCE and AgNPs presented 33.2, 36.1% inhibitory activity on tyrosinase, respectively. In silico assay demonstrated interaction between steroidal saponins, DNA or tyrosinase. SaCE exhibited antitumor action against various human tumor cells. Data demonstrated that extracts SaCE alone and AgNPs synthesized from SaCE presented biological properties of interest for application in new therapeutic formulations in medicine.