鼻咽癌（NPC）是一种常见的头颈部恶性肿瘤。免疫相关甲基化生物标志物的鉴定可能有助于鼻咽癌的检测和预后评估。构建了基于WGCNA的共甲基化网络来识别鼻咽癌和免疫细胞相关的模块。结合ImmPort数据库中的差异表达基因（DEG）和免疫相关基因，获得候选免疫相关甲基化基因（IRMG）。我们的组合分析鉴定出12个IRMG。其中，CCL28、CSK、PRKCB的甲基化和mRNA表达均与B细胞的浸润相关。 CD1D、CR2 和 GDF10 是有利的标记。去甲基化实验验证了 NPC 中 GDF10、PRKCB、SLC40A1 和 TGFBR3 的下调是由启动子高甲基化引起的。此外，还开发了一个诊断模型，并表现出很高的判别准确性。这些结果提供了一组可能有助于鼻咽癌的诊断和预后的免疫相关甲基化生物标志物。© 2023 Wiley periodicals LLC。

Nasopharyngeal carcinoma (NPC) is a common malignancy occurring in the head and neck. Identification of immune-related methylated biomarkers might be helpful for NPC detection and prognostic evaluation.A co-methylation network based on WGCNA was constructed to identify modules associated with NPC and immune cells. In combination with differentially expressed genes (DEGs) and immune-related genes from ImmPort database, the candidate immune-related methylated genes (IRMGs) were obtained.Our combined analysis identified 12 IRMGs. Among them, both the methylation and mRNA expression of CCL28, CSK, and PRKCB were correlated with the infiltration of B cells. CD1D, CR2, and GDF10 were favorable markers. Demethylation experiments validated that downregulation of GDF10, PRKCB, SLC40A1, and TGFBR3 in NPC resulted from promoter hypermethylation. Additionally, a diagnostic model was developed and exhibited high discriminative accuracy.These results provided a group of immune-related methylated biomarkers that may help with the diagnosis and prognosis of NPC.© 2023 Wiley Periodicals LLC.