基因治疗是一种很有前途的方法，它可以使用阳离子或可电离的脂质纳米颗粒递送 mRNA、小干扰 RNA 和质粒 DNA，从而在体外引发治疗作用。在本研究中，采用内部开发的新型挤出式喷雾多吸附液滴重置技术 (SMART)，用于体外癌细胞中绿色荧光蛋白 (GFP) 质粒 DNA 的制备、表征和转染能力。结果显示，pDNA (GFP) 100% 封装在约 150 nm (N/P 5) 的 LNP 中，表明使用 SMART 技术开发的工艺是一致的，可用于商业应用。

Gene therapy is a promising approach with delivery of mRNA, small interference RNA, and plasmid DNA to elicit a therapeutic action in vitro using cationic or ionizable lipid nanoparticles. In the present study, a novel extrusion-based Sprayed Multi Adsorbed-droplet Reposing Technology (SMART) developed in-house was employed for the preparation, characterization, and transfection abilities of the green fluorescence protein (GFP) plasmid DNA in cancer cells in vitro. The results showed 100% encapsulation of pDNA (GFP) in LNPs of around 150 nm (N/P 5) indicating that the processes developed using SMART technology are consistent and can be utilized for commercial applications.