绿色纳米技术被认为是构建具有显着抗癌活性的功能材料的一种有前途的方法，同时克服了传统合成工艺复杂和有机溶剂消耗高的缺点。因此，在本研究中，我们首次报道了功能化5-氟尿嘧啶和姜黄素共载溶菌酶-透明质酸复合胶体纳米粒子（5-Fu/Cur@LHNPs）的合理设计和绿色合成，以更好地靶向结直肠癌治疗最大限度地减少副作用。功能化的5-Fu/Cur@LHNPs具有稳定的粒径（126.1nm）、优异的均匀性（PDI=0.1）、良好的胶体稳定性和优异的再分散性。体外细胞实验表明，5-Fu/Cur@LHNPs的细胞摄取显着提高，并进一步促进HCT-116细胞更高的凋亡率。与对照组相比，5-Fu/Cur@LHNPs制剂组实现了对结直肠肿瘤生长的有效抑制（60.1%）。构建 5-Fu/Cur@LHNPs 的无酒精自组装方法对于转化化疗药物而言简单且安全，也可促进治疗结直肠癌的更强大的递送系统。版权所有 © 2023。由 Elsevier B.V. 出版。

Green nanotechnology is considered a promising method to construct functional materials with significant anticancer activity, while overcoming the shortcomings of traditional synthesis process complexity and high organic solvents consumption. Thus, in this study, we report for the first time the rational design and green synthesis of functionalized 5-fluorouracil and curcumin co-loaded lysozyme-hyaluronan composite colloidal nanoparticles (5-Fu/Cur@LHNPs) for better targeted colorectal cancer therapy with minimized side effects. The functionalized 5-Fu/Cur@LHNPs exhibit stabilized particle size (126.1 nm) with excellent homogeneity (PDI = 0.1), favorable colloidal stabilities, and excellent re-dispersibility. In vitro cell experiments illustrate that the cellular uptake of 5-Fu/Cur@LHNPs was significantly improved and further promoted a higher apoptosis ratio of HCT-116 cells. Compared with the control group, the 5-Fu/Cur@LHNPs formulation group achieved effective inhibition (60.1 %) of colorectal tumor growth. The alcohol-free self-assembly method to construct 5-Fu/Cur@LHNPs is simple and safe for a translational chemotherapy drug, also to promote more robust delivery systems for treating colorectal cancer.Copyright © 2023. Published by Elsevier B.V.