白血病是骨髓中白细胞异常增殖，导致血液和骨髓中异常白血病细胞大量积聚。痔疮是直肠或肛门区域的静脉扩张和肿胀。然而，CALM3与白血病和痔疮之间的关系仍不清楚。痔疮数据集GSE154650和白血病数据集GSE26294从GPL20301和GPL571生成的GEO数据库中下载。使用R包limma筛选差异表达基因（DED）。进行加权基因共表达网络分析（WGCNA）。进行了蛋白质-蛋白质相互作用（PPI）网络的构建和分析、功能富集分析、基因集富集分析（GSEA）和比较毒理基因组数据库（CTD）分析。 TargetScan用于筛选调节中央DEG的miRNA。通过蛋白质印迹基础细胞测定对其进行验证。共鉴定出 125 个 DEG。根据GO分析，它们主要富集于小分子分解代谢过程、皮肤发育和趋化因子受体结合。 KEGG分析结果显示靶细胞主要富集于细胞因子与细胞因子受体的相互作用以及丁酸代谢。 GSEA 分析结果表明小分子分解代谢过程、皮肤发育和趋化因子受体结合的富集。确定了六个核心基因（CALM3、ACE2、PPARGC1A、XCR1、CFTR、PRKCA）。我们发现核心基因CALM3在痔疮样本中高表达，在白血病样本中低表达，在正常样本中低表达，可能在痔疮和白血病中发挥调节作用。免疫浸润结果显示 T_cells_CD4_memory_resting 比例较高，且与 T_cells_CD8 相关。 WB实验验证了这一结果。 CALM3在白血病中表达较低，表达越低，预后越差。 CALM3在痔疮中高表达，表达越高，预后越差。Copyright © 2023 the Author(s).由 Wolters Kluwer Health, Inc. 出版

Leukemia is an abnormal proliferation of white blood cells in the bone marrow, resulting in a large accumulation of abnormal leukemia cells in the blood and bone marrow. Hemorrhoids are dilated and swollen veins in the rectum or anal area. However, the relationship between CALM3 and leukemia and hemorrhoids remains unclear. The hemorrhoids dataset GSE154650 and leukemia dataset GSE26294 were downloaded from GEO databases generated by GPL20301 and GPL571.The R package limma was used to screen differentially expressed genes (DEDs). Weighted gene co-expression network analysis (WGCNA) was performed. The construction and analysis of protein-protein interaction (PPI) network, functional enrichment analysis, Gene Set Enrichment Analysis (GSEA) and comparative toxicogenomics database (CTD) analysis were performed. TargetScan was used to screen miRNAs regulating central DEGs. It was verified by western blot basic cell assay. A total of 125 DEGs were co-identified. According to the GO analysis, they are mainly enriched in small molecule catabolic processes, skin development, and chemokine receptor binding. The KEGG analysis results show that the target cells are mainly enriched in the interaction of cytokines and cytokine receptors, as well as butyric acid metabolism. The GSEA analysis results indicate enrichment in small molecule catabolic processes, skin development, and chemokine receptor binding. Six core genes (CALM3, ACE2, PPARGC1A, XCR1, CFTR, PRKCA) were identified. We found that the core gene CALM3 is highly expressed in hemorrhoid samples, low in leukemia samples, and has low expression in normal samples, which may play a regulatory role in hemorrhoids and leukemia. Immunoinfiltration results showed a higher proportion of T_cells_CD4_memory_resting and a correlation with T_cells_CD8. WB experiment verified the result. CALM3 expression is low in leukemia, and the lower the expression is, the worse the prognosis is. CALM3 is highly expressed in hemorrhoids, and the higher the expression, the worse the prognosis.Copyright © 2023 the Author(s). Published by Wolters Kluwer Health, Inc.