机械力在分子和细胞水平的生物过程中发挥着至关重要的作用，对癌症、心血管疾病和 COVID-19 等多种疾病产生重大影响。动态力谱 (DFS) 技术的最新进展使得能够以高分辨率应用和测量力和位移，为了解这些疾病背后的机械途径提供了重要的见解。在 DFS 技术中，生物膜力探针 (BFP) 因其能够以皮牛顿和纳米分辨率测量键动力学和细胞机械传感而脱颖而出。在这里，全面概述了经典的 BFP-DFS 设置，并强调了关键进展，包括双生物膜力探针 (dBFP) 和荧光生物膜力探针 (fBFP) 的开发。 BFP-DFS 使我们能够研究活细胞上的动态键行为，并显着增强对特定配体-受体轴介导的细胞机械传感的理解。深入研究了 BFP-DFS 对癌症生物学、血栓形成和炎症领域的贡献，探索其阐明新治疗发现的潜力。此外，预计未来的 BFP 升级旨在提高产量和可行性，强调其在细胞机械生物学领域日益增长的重要性。尽管 BFP-DFS 仍然是一种利基研究模式，但它对不断扩大的细胞力生物学领域的影响是巨大的。© 2023 作者。河南大学和约翰·威利出版的《探索》

Mechanical forces play a vital role in biological processes at molecular and cellular levels, significantly impacting various diseases such as cancer, cardiovascular disease, and COVID-19. Recent advancements in dynamic force spectroscopy (DFS) techniques have enabled the application and measurement of forces and displacements with high resolutions, providing crucial insights into the mechanical pathways underlying these diseases. Among DFS techniques, the biomembrane force probe (BFP) stands out for its ability to measure bond kinetics and cellular mechanosensing with pico-newton and nano-meter resolutions. Here, a comprehensive overview of the classical BFP-DFS setup is presented and key advancements are emphasized, including the development of dual biomembrane force probe (dBFP) and fluorescence biomembrane force probe (fBFP). BFP-DFS allows us to investigate dynamic bond behaviors on living cells and significantly enhances the understanding of specific ligand-receptor axes mediated cell mechanosensing. The contributions of BFP-DFS to the fields of cancer biology, thrombosis, and inflammation are delved into, exploring its potential to elucidate novel therapeutic discoveries. Furthermore, future BFP upgrades aimed at improving output and feasibility are anticipated, emphasizing its growing importance in the field of cell mechanobiology. Although BFP-DFS remains a niche research modality, its impact on the expanding field of cell mechanobiology is immense.© 2023 The Authors. Exploration published by Henan University and John Wiley & Sons Australia, Ltd.