光动力疗法（PDT）已成为口腔癌治疗的一种有前景的策略。维替泊芬是一种强效光敏剂，广泛用于治疗黄斑变性。然而，很少有工作报道其在口腔癌治疗中的潜力。在这项研究中，我们介绍了一种基于维替泊芬的纳米光敏剂的创新方法，该纳米光敏剂是通过利用巨噬细胞膜涂覆维替泊芬沸石咪唑酯骨架8（ZIF -8) 用于针对口腔癌的有效光动力疗法。评估了纳米颗粒的特性，包括尺寸、zeta 电位和 PDI。使用 CAL-27 细胞进行细胞摄取研究。此外，还观察到体外和体内的抑制作用，确保了生物安全。抗癌功效的评估包括肿瘤体积测量、组织学分析和免疫组织化学染色。体外实验表明纳米光敏剂在口腔癌细胞中显示出有效的细胞摄取。在激光照射下，纳米光敏剂诱导活性氧（ROS）的产生，导致癌细胞凋亡。体内实验表明，细胞膜涂层增强了纳米光敏剂的循环时间。此外，在荷瘤小鼠模型中，纳米光敏剂对癌细胞的特异性也通过细胞膜伪装结构得到提高，在激光照射下通过光动力效应显着抑制肿瘤生长。我们的研究结果表明，负载维替泊芬的巨噬细胞膜包被的 ZIF-8 纳米颗粒在口腔癌治疗中具有有效光动力疗法的潜力。这种纳米系统通过将 PDT 的细胞毒性作用与免疫系统的激活相结合，有望实现协同癌症治疗，提供一种对抗癌症的新颖治疗策略。© 2023 Yu 等人。

Photodynamic therapy (PDT) has emerged as a promising strategy for oral cancer treatment. Verteporfin is a powerful photosensitizer and widely used in the treatment of macular degeneration. However, rare work has reported its potential in the treatment of oral cancer.In this study, we introduce an innovative approach of nano-photosensitizer based on Verteporfin, which was prepared by utilizing macrophage membrane to coat Verteporfin-loaded zeolitic imidazolate framework 8 (ZIF-8) for effective photodynamic therapy against oral cancer. Nanoparticle characteristics were assessed including size, zeta potential, and PDI. Cellular uptake studies were conducted using CAL-27 cells. Furthermore, inhibitory effects in both in vitro and in vivo settings were observed, ensuring biosafety. Assessment of anticancer efficacy involved tumor volume measurement, histological analyses, and immunohistochemical staining.In vitro experiments indicated that the nano-photosensitizer showed efficient cellular uptake in the oral cancer cells. Upon the laser irradiation, the nano-photosensitizer induced the generation of reactive oxygen species (ROS), leading to cancer cell apoptosis. The in vivo experiments indicated that the coating with cell membranes enhanced the circulation time of nano-photosensitizer. Moreover, the specificity of the nano-photosensitizer to the cancer cells was also improved by the cell membrane-camouflaged structure in the tumor-bearing mouse model, which inhibited the tumor growth significantly by the photodynamic effect in the presence of laser irradiation.Overall, our findings demonstrate the potential of macrophage membrane-coated ZIF-8-based nanoparticles loaded with Verteporfin for effective photodynamic therapy in oral cancer treatment. This nano-system holds promise for synergistic cancer therapy by combining the cytotoxic effects of PDT with the activation of the immune system, providing a novel therapeutic strategy for combating cancer.© 2023 Yu et al.