快速、准确地识别肿瘤代谢标志物对于肿瘤的早期诊断和个体化治疗具有重要意义。这里，通过聚合物聚乙烯吡咯烷酮原位生长域限制的Pt量子点，开发了一种稳定的单分散亚纳米铂（Pt）材料作为高效纳米酶，其过氧化物酶比活性高达20.86 U mg-1 。此外，通过甲硅烷基化反应测定了大量Pt负载SiO2（Pt-SiO2）的合成，并用于人甲胎蛋白（AFP）的肉眼比色测试。特别地，免疫孵育过程发生在预先准备好的微孔板中。在目标 AFP 存在的情况下构建了基于纳米酶的免疫模型，并与外源过氧化氢和显色底物四甲基联苯胺发生显色反应。通过优化实验条件，AFP的动态工作响应范围为0.05-20ng mL-1，检测限为38.7pg mL-1。这项工作提供了设计高效的基于纳米酶的酶联免疫层析平台的新策略，以满足替代天然酶的实际使用。© 2023 John Wiley

Rapid and accurate identification of tumor metabolic markers is important for early tumor diagnosis and individualized treatment. Here, a stable monodisperse sub-nanometer platinum (Pt) material was developed as a highly efficient nanozyme with a specific activity of peroxidase as high as 20.86 U mg-1 through the growth of in situ domain-limited Pt quantum dots via the polymer polyvinylpyrrolidone. Further, the synthesis of large quantities of Pt-loaded SiO2 (Pt-SiO2 ) was determined by silylation reaction and used for naked eye colorimetric testing of human alpha-fetoprotein (AFP). In particular, the immunization incubation process occurred in preprepared microplates. A nanozyme-based immunomodel was constructed in the presence of the target AFP, and a chromogenic reaction occurred with exogenous hydrogen peroxide and the chromogenic substrate tetramethylbenzidine. On optimization of experimental conditions, the dynamic working response range for AFP was found to be 0.05-20 ng mL-1 , with a limit of detection of 38.7 pg mL-1 . This work provides a new strategy to design efficient nanozyme-based enzyme-linked immunochromatographic platforms to meet the practical use of replacing natural enzymes.© 2023 John Wiley & Sons Ltd.