植物中存在的酚类化合物已显示出多种生物学特性，例如抗氧化剂、抗肿瘤、心脏保护和抗增殖。另一方面，广泛用于治疗乳腺癌的化疗药物阿霉素通常表现出与氧化应激相关的慢性心脏毒性。因此，我们的目的是研究富含酚类化合物的提取物（PCEE）与阿霉素对乳腺癌的影响。为了实现这一目标，对山核桃壳（Carya illinoinensis）的粗提物进行 SPE-C18 柱纯化后，所得 PCEE 用于评估对人乳腺癌细胞系 MDA-MB-231 的细胞毒性和抗氧化特性，以及正常仓鼠卵巢细胞系 CHO-K1。 PCEE 对两种细胞系均具有选择性细胞毒性，MDA-MB-231 的 IC50 值（约 26.34 mg/L）低于 CHO-K1 的 IC50 值（约 55.63 mg/L）。作为一种细胞毒性机制，PCEE 通过 MDA-MB-231 细胞中 G2/M 细胞周期停滞来抑制细胞生长。同时，抗氧化活性研究表明，PCEE 具有细胞保护作用，由多柔比星引起的氧化应激细胞中 ROS 的产生减少就证明了这一点。结果强调 PCEE 作为一种潜在的抗肿瘤剂，从而重新评估其作为农工业残留物的价值。© 2023 John Wiley

Phenolic compounds present in plants have demonstrated several biological properties such as antioxidant, antitumor, cardioprotective, and antiproliferative. On the other hand, doxorubicin, a chemotherapeutic widely used to treat breast cancer, usually exhibits chronic cardiotoxicity associated with oxidative stress. Therefore, we aimed to study the effects of phenolic compound-enriched extract (PCEE) with doxorubicin in breast cancer. To achieve this, after an SPE-C18 -column purification process of crude extracts obtained from pecan nutshells (Carya illinoinensis), the resulting PCEE was used to evaluate the cytotoxicity and antioxidant properties against the human breast cancer cell line MDA-MB-231 and the normal-hamster ovary cell line CHO-K1. PCEE was selectively cytotoxic against both cell lines, with an IC50 value (≈26.34 mg/L) for MDA-MB-231 lower than that obtained for CHO-K1 (≈55.63 mg/L). As a cytotoxic mechanism, PCEE inhibited cell growth by G2/M cell cycle arrest in MDA-MB-231 cells. Simultaneously, the study of the antioxidant activity showed that PCEE had a cytoprotective effect, evidenced by reduced ROS production in cells with oxidative stress caused by doxorubicin. The results highlight PCEE as a potential antitumor agent, thus revaluing it as an agro-industrial residue.© 2023 John Wiley & Sons Ltd.