评估使用传统药用植物的风险和益处对于很大一部分人口来说至关重要，特别是在越南北部。斑马鱼越来越多地用作简单的脊椎动物模型，用于测试化合物的毒性和生理效应，特别是在发育方面。在这里，我们测试了从越南北部收集的流行药用植物中提取的 12 种乙醇提取物，了解它们对受精后前 4 天内斑马鱼生存和发育的影响。我们更详细地描述了它们对表皮、孵化、生长、坏死、身体弯曲、血管生成、骨骼发育以及主要增加的运动行为的影响。最后，我们通过对肌动蛋白丝进行染色并进行全基因组基因表达分析，确认了十大功劳提取物对表皮的影响。此外，我们发现这种提取物还抑制小鼠胚胎成纤维细胞的细胞迁移。最后，我们分析了十大功劳提取物的化学成分，并测试了其主要成分的功效。总之，我们表明传统药用植物提取物能够不同程度地影响斑马鱼早期生命阶段的发育。此外，我们还发现，导致外延延迟的提取物也能抑制哺乳动物细胞迁移，这表明这种效应可以作为鉴定抑制癌症转移的提取物的初步测试。版权所有：© 2023 Tran 等人。这是一篇根据知识共享署名许可条款分发的开放获取文章，允许在任何媒体上不受限制地使用、分发和复制，前提是注明原始作者和来源。

Evaluating the risks and benefits of using traditional medicinal plants is of utmost importance for a huge fraction of the human population, in particular in Northern Vietnam. Zebrafish are increasingly used as a simple vertebrate model for testing toxic and physiological effects of compounds, especially on development. Here, we tested 12 ethanolic extracts from popular medicinal plants collected in northern Vietnam for their effects on zebrafish survival and development during the first 4 days after fertilization. We characterized more in detail their effects on epiboly, hatching, growth, necrosis, body curvature, angiogenesis, skeletal development and mostly increased movement behavior. Finally, we confirm the effect on epiboly caused by the Mahonia bealei extract by staining the actin filaments and performing whole genome gene expression analysis. Further, we show that this extract also inhibits cell migration of mouse embryo fibroblasts. Finally, we analyzed the chemical composition of the Mahonia bealei extract and test the effects of its major components. In conclusion, we show that traditional medicinal plant extracts are able to affect zebrafish early life stage development to various degrees. In addition, we show that an extract causing delay in epiboly also inhibits mammalian cell migration, suggesting that this effect may serve as a preliminary test for identifying extracts that inhibit cancer metastasis.Copyright: © 2023 Tran et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.