提高癌细胞中的活性氧（ROS）水平以引起大分子损伤和细胞死亡是一种有前途的抗癌治疗策略。观察到电磁场 (EMF) 会提高细胞内 ROS 并导致癌细胞死亡，因此我们开发了一种新型便携式可穿戴 EMF 设备，该设备可产生旋转振荡磁场 (sOMF)，选择性地杀死癌细胞，同时在体外不伤害正常细胞并使其缩小GBM肿瘤通过一种新的机制在体内发挥作用。在这里，我们描述了 sOMF 刺激的精确配置和时间安排，这些刺激由于两种类型的人类神经胶质瘤细胞中超氧化物的急剧增加而产生细胞毒性。我们还发现抗氧化剂 Trolox 可以逆转 sOMF 对神经胶质瘤细胞的细胞毒性作用，表明 ROS 在产生这种作用中发挥着因果作用。我们的研究结果阐明了磁刺激的物理原理与其抗癌作用机制之间的联系，促进了针对 GBM 和其他神经胶质瘤的潜在新型安全无创设备治疗的开发。© 2023。作者。

Raising reactive oxygen species (ROS) levels in cancer cells to cause macromolecular damage and cell death is a promising anticancer treatment strategy. Observations that electromagnetic fields (EMF) elevate intracellular ROS and cause cancer cell death, have led us to develop a new portable wearable EMF device that generates spinning oscillating magnetic fields (sOMF) to selectively kill cancer cells while sparing normal cells in vitro and to shrink GBM tumors in vivo through a novel mechanism. Here, we characterized the precise configurations and timings of sOMF stimulation that produce cytotoxicity due to a critical rise in superoxide in two types of human glioma cells. We also found that the antioxidant Trolox reverses the cytotoxic effect of sOMF on glioma cells indicating that ROS play a causal role in producing the effect. Our findings clarify the link between the physics of magnetic stimulation and its mechanism of anticancer action, facilitating the development of a potential new safe noninvasive device-based treatment for GBM and other gliomas.© 2023. The Author(s).